FlyBase Allele Report: Dmel\dila[81]

General Information

Symbol

Dmel\dila⁸¹

Species

D. melanogaster

Name

FlyBase ID

FBal0262490

Feature type

allele

Associated gene

Dmel\dila

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Ma and Jarman, 2011)

Mutagen

Delta2-3 transposase

(Ma and Jarman, 2011)

Progenitor genotype

P{GT1}CG30001^BG02674

(Ma and Jarman, 2011)

Caused by aberration

Df(2R)dila⁸¹

Cytology

Description

Imprecise excision of P{GT1}CG30001^BG02674 resulting in a 2 kb deletion in which some of the CG30001 5'UTR and all/most of the DNA encoding the dila C-terminal coiled-coil domain is removed.

(Ma and Jarman, 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:9,823,017..9,825,017 [-]

Comment:

Approximate endpoints of a ~2.0 kb deletion resulting from the imprecise excision of P{GT1}CG30001^BG02674.

(Ma and Jarman, 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

bang sensitive | recessive

(Ma and Jarman, 2011)

short lived | recessive

(Ma and Jarman, 2011)

uncoordinated | recessive

(Ma and Jarman, 2011)

Phenotype Manifest In

axonemal dynein complex

(Ma and Jarman, 2011)

axonemal microtubule

(Ma and Jarman, 2011)

cilium

(Ma and Jarman, 2011)

motile cilium

(Ma and Jarman, 2011)

spermatozoon

(Ma and Jarman, 2011)

Detailed Description

Statement

Reference

Homozygotes reach the adult stage and these mutant flies show uncoordinated behaviour. They have a dramatically reduced climbing ability in bang tests compared with wild type controls. They are incapable of standing or righting themselves. They die very soon after eclosion.

Cilia of chordotonal neurons within Johnston's organ (in the adult antenna) show a range of ultrastructural defects in dila⁸¹ mutants. Some cilia are shortened; others are present but usually have a disorganised axonemal structure, often with missing microtubule doublets, or are occasionally grossly abnormal in structure or orientation. In addition, the doublets mostly lack their associated dynein arms. In contrast to the ciliary compartment, basal sections show no obvious defects in the ciliary rootlet or basal bodies and their associated fibrous structures. Likewise, no obvious defects are seen in the putative 'transition zone' beyond the basal bodies.

Chordotonal neuronal cilia are also missing or shortened in dila⁸¹ embryos and larvae.

dila⁸¹ male flies in which peripheral nervous system defects are substantially rescued by dila^{Scer\UAS.N.T:Zzzz\FLAG} or dila^{Scer\UAS.C.T:Zzzz\FLAG} expression show a very low level of fertility.

dila⁸¹ testes are of normal size and shape but few, if any, mature motile sperm are evident in 2-day old testes. No defects in meiotic cysts and a very low level of defects in onion-stage spermatocytes are seen. However, during the elongating stage, sperm nuclei show substantial disorganization: some nuclei are misorientated within the nuclear clusters and others are dispersed along the flagellar bundles. In addition, substantial disorganization of the flagellar bundles is observed and basal body markers are mislocalized. There are also individualization defects with many sperm tails sharing syncitial cytoplasm or lacking surrounding cytoplasmic membrane. Many flagella are separated from their mitochondrial derivatives and mixed axoneme polarities are observed. Despite these defects, flagellar axoneme structure appears intact.

Neuronal cell division is normal in dila⁸¹ embryos.

(Ma and Jarman, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Cby¹, dila⁸¹ has male sterile phenotype

(Vieillard et al., 2016)

Cby¹, dila⁸¹ has uncoordinated | adult stage phenotype

(Vieillard et al., 2016)

Cby¹, dila⁸¹ has abnormal neuroanatomy | adult stage phenotype

(Vieillard et al., 2016)

Phenotype Manifest In

Other

Statement

Reference

Cby¹, dila⁸¹ has spermatocyte | adult stage phenotype

(Vieillard et al., 2016)

Cby¹, dila⁸¹ has centriole | male | adult stage phenotype

(Vieillard et al., 2016)

Cby¹, dila⁸¹ has axoneme | male | adult stage phenotype

(Vieillard et al., 2016)

Cby¹, dila⁸¹ has chordotonal organ | adult stage phenotype

(Vieillard et al., 2016)

Cby¹, dila⁸¹ has sensory neuron | adult stage phenotype

(Vieillard et al., 2016)

Cby¹, dila⁸¹ has spermatocyte cyst | adult stage phenotype

(Vieillard et al., 2016)

Cby¹, dila⁸¹ has ciliary cap phenotype

(Vieillard et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

dila⁸¹;Cby¹ double mutant flies are completely uncoordinated and show ultrastructural defects in chordotonal organ of the second antennal segment: cilia are mostly missing - the few remaining ones are severely disorganized and although both basal bodies (proximal and distal) are present, no transition zone is formed. The males are completely sterile, produce no mature sperm, their sperm cysts fail to elongate (but the overall size of their testes is not reduced) and show severe ultrastructural defects: Most of the axonemes are missing or damaged, the centrioles of late spermatocytes do not form a primary cilium-like structure, fail to dock to the plasma membrane, have an abnormal orientation in the cell, no ciliary cap is observed in these spermatocytes and premature axoneme elongation occurs.

(Vieillard et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dila⁸¹ is rescued by dila^{UAS.N.Tag:FLAG}/Scer\GAL4^sca-109-68

(Ma and Jarman, 2011)

dila⁸¹ is rescued by Scer\GAL4^elav.PU/dila^{UAS.N.Tag:FLAG}

(Ma and Jarman, 2011)

dila⁸¹ is rescued by dila^{UAS.C.Tag:FLAG}/Scer\GAL4^sca-109-68

(Ma and Jarman, 2011)