FlyBase Allele Report: Dmel\aPKC[GL00007]

General Information

Symbol

Dmel\aPKC^GL00007

Species

D. melanogaster

Name

FlyBase ID

FBal0262499

Feature type

allele

Associated gene

Dmel\aPKC

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00007}

Also Known As

GL00007, UAS-aPKC RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00007}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00007

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Sopko et al., 2014)

increased cell death | oogenesis, with Scer\GAL4^nanos.PG

(Wang et al., 2019)

Phenotype Manifest In

female germline stem cell, with Scer\GAL4^nanos.PG

(Wang et al., 2019)

germarium, with Scer\GAL4^nanos.PG

(Wang et al., 2019)

spermatid cyst, with Scer\GAL4^{VP16.nanos.UTR}

(Xu et al., 2014)

spermatid cyst, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Xu et al., 2014)

testis, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Xu et al., 2014)

Detailed Description

Statement

Reference

The expression of aPKC^GL00007 under the control of Scer\GAL4^nos.PG leads to germline degeneration in the germarium.

(Wang et al., 2019)

Females heterozygous for aPKC^GL00007 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to aPKC^GL00007 males results in: no eggs; egg/embryonic phenotype: no vitellogenic egg chambers; oogenesis defect.

(Sopko et al., 2014)

Testes of males expressing aPKC^GL00007 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} sometimes contain misoriented spermatid cysts where the clustered spermatid nuclei are found apically (rather than basally). When expression is driven by the triple "MTD" driver line (Scer\GAL4^nos.PG, Scer\GAL4^{nos.UTR.T:Hsim\VP16} and Scer\GAL4^{otu.T:Hsim\VP16}) 100% of testes have multiple mis-oriented spermatid cysts and the testes are shorter than normal.

(Xu et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

aPKC^GL00007/Scer\GAL4^Bx-MS1096 is a non-enhancer of neoplasia | larval stage phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

NOT Suppressor of

Statement

Reference

aPKC^GL00007/Scer\GAL4^Bx-MS1096 is a non-suppressor of neoplasia | larval stage phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

aPKC^GL00007/Scer\GAL4^Bx-MS1096 is a non-enhancer of wing disc phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

NOT Suppressor of

Statement

Reference

aPKC^GL00007/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing disc phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35140

y¹ sc^* v¹ sev²¹; P{TRiP.GL00007}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

aPKC^GL00007

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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