The percentage of polycyclic pulses in courtship song is significantly higher in adults expressing Camta^GLV21048 under the control of Scer\GAL4^{Camta-MI04570-TG4.1}, Scer\GAL4^elav-C155, Scer\GAL4^nSyb.PU, Scer\GAL4^fru.PU, but not Scer\GAL4^repo.PU (in combination with Dicer-2, for an efficient RNAi), Scer\GAL4^dsx.KI (in combination with Dicer-2, for an efficient RNAi), or Scer\GAL4^VT040556, as compared to controls. This phenotype is also observed upon clonal expression under the control of Scer\GAL4^fru.PU if restricted to the brain, but not if excluded from the brain (clonality achieved by Scer\FLP1^{FLPo.oc.Tag:NLS(SV40-largeT)}-induced Gal80 flip-out clones); similar clonal expressions under the control of Scer\GAL4^dsx.KI do not induce such phenotype whether restricted to or excluded from the brain. The phenotype is also observed upon clonal expression in fru-positive clones (clonality achieved by Scer\FLP1^fru-induced Gal80 flip-out clones) when under the control of Scer\GAL4^p52a, but not Scer\GAL4^VT040556 or Scer\GAL4^NP2631.