FlyBase Allele Report: Dmel\Cdk2[HMS01543]

General Information

Symbol

Dmel\Cdk2^HMS01543

Species

D. melanogaster

Name

FlyBase ID

FBal0263446

Feature type

allele

Associated gene

Dmel\Cdk2

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01543}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01543}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01543

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Sopko et al., 2014)

Phenotype Manifest In

fat cell | third instar larval stage, with Scer\GAL4^Cg.PA

(Sun et al., 2019)

microtubule | third instar larval stage, with Scer\GAL4^Cg.PA

(Sun et al., 2019)

nucleus | third instar larval stage, with Scer\GAL4^Cg.PA

(Sun et al., 2019)

Detailed Description

Statement

Reference

Expressing Cdk2^HMS01543 under the control of Scer\GAL4^Cg.PA leads to adipocytes exhibiting reduced perinuclear microtubule concentration and decreased ploidy, without preventing differentiation, as they are still full of lipid droplets. Decreased ploidy is also observed upon conditional expression from L2 onwards (temporal control of expression using Gal80[ts]).

(Sun et al., 2019)

Females heterozygous for Cdk2^HMS01543 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to Cdk2^HMS01543 males results in: no eggs; egg/embryonic phenotype: no vitellogenic egg chambers; oogenesis defect.

(Sopko et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Cdk2^HMS01543, Scer\GAL4^Cg.PA is a suppressor of fat cell | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^JF02971

(Sun et al., 2019)