FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\nclbUASp.cCa
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General Information
Symbol
Dmel\nclbUASp.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae construct a of Casper
FlyBase ID
FBal0263560
Feature type
allele
Associated gene
Dmel\nclb
Associated Insertion(s)
Carried in Construct
P{UASp-nclb.C}
Key Links
Transgenic product class
wild_type
(Casper et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Casper et al., 2011)
Mutagen
in vitro construct
(Casper et al., 2011)
Progenitor genotype
Carried in construct
P{UASp-nclb.C}
Cytology
Description

UASp regulatory sequences drive expression of nclb coding sequences.

(Casper et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
nclb
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      nclbUASp.cCa/Scer\GAL4VP16.nanos.UTR rescues nclb2/nclb1

      (Casper et al., 2011)

      nclbUASp.cCa/Scer\GAL4αTub84B.PL rescues nclb2/nclb1

      (Casper et al., 2011)

      nclbUASp.cCa/Scer\GAL4da.PU rescues nclb2/nclb1

      (Casper et al., 2011)
      Comments

      Expression of nclbScer\UAS.P\T.cCa in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is sufficient to rescue the germline loss phenotype observed in nclb1/nclb2 mutants. Rescued testes have a wild-type appearance, with numerous germline stem cells associated with the hub and ample numbers of differentiating spermatogenic cysts. In addition, these rescued males have elongated spermatids and are completely fertile.

      Expression of nclbScer\UAS.P\T.cCa under the control of Scer\GAL4αTub84B.PL rescues the germline and fertility defects found in nclb1/nclb2 mutants.

      Expression of nclbScer\UAS.P\T.cCa under the control of Scer\GAL4αTub84B.PL rescues the lethality found in homozygous nclb2 mutants. Fertility is not rescued in these mutants.

      Expression of nclbScer\UAS.P\T.cCa under the control of Scer\GAL4αTub84B.PL fails to rescue nclb1/nclb2 mutant stage 5 egg chamber arrest and fertility defects. More general expression using Scer\GAL4αTub84B.PL, also fails to completely rescue the stage 5 nurse endoreplication phenotype but does partially rescue fertility. Expression with Scer\GAL4da.G32, which expresses strongly in both the germline and the soma, is sufficient to rescue both the egg chamber arrest and fertility defects of nclb1/nclb2 mutants.

      (Casper et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      nclbScer\UAS.P\T.cCa
      nclbUASp.cCa
      Name Synonyms
      Saccharomyces cerevisiae construct a of Casper
      Secondary FlyBase IDs
        References (1)