FlyBase Allele Report: Dmel\nclb[1]

General Information

Symbol

Dmel\nclb¹

Species

D. melanogaster

Name

FlyBase ID

FBal0263561

Feature type

allele

Associated gene

Dmel\nclb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Casper et al., 2011)

Progenitor genotype

Cytology

Description

Imprecise excision of P{EPgy2}CG6751^EY10712 results in an internal deletion within P{EPgy2}CG6751^EY10712.

(Casper et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage (with nclb²)

(Liu et al., 2017)

abnormal endomitotic cell cycle

(Casper et al., 2011)

abnormal endomitotic cell cycle (with nclb²)

(Casper et al., 2011)

abnormal size | adult stage (with nclb²)

(Liu et al., 2017)

decreased body size | larval stage (with nclb²)

(Liu et al., 2017)

decreased body size | larval stage | recessive

(Liu et al., 2017)

decreased body size | pupal stage (with nclb²)

(Liu et al., 2017)

partially lethal - majority die (with nclb²)

(Liu et al., 2017)

sterile

(Casper et al., 2011)

viable

(Casper et al., 2011)

Phenotype Manifest In

egg chamber | oogenesis stage S5

(Casper et al., 2011)

egg chamber | oogenesis stage S5 (with nclb²)

(Casper et al., 2011)

female germline cell

(Casper et al., 2011)

female germline cell (with nclb²)

(Casper et al., 2011)

individualization stage spermatid

(Casper et al., 2011)

individualization stage spermatid (with nclb²)

(Casper et al., 2011)

macrochaeta | adult stage (with nclb²)

(Liu et al., 2017)

male germline cell

(Casper et al., 2011)

male germline cell (with nclb²)

(Casper et al., 2011)

nurse cell | oogenesis stage S5

(Casper et al., 2011)

nurse cell | oogenesis stage S5 (with nclb²)

(Casper et al., 2011)

ovary

(Casper et al., 2011)

ovary (with nclb²)

(Casper et al., 2011)

testis

(Casper et al., 2011)

testis (with nclb²)

(Casper et al., 2011)

Detailed Description

Statement

Reference

nclb¹/nclb² transheterozygotes are semi-viable, exhibit a significant delay in pupation, coupled with a significantly smaller body size/volume in both larvae and pupae, as compared to controls. nclb¹ homozygotes also show a small larval body size, as compared to controls.

(Liu et al., 2017)

nclb¹ mutant adult testes exhibit a reduced number of germ cells and an absence of mature sperm.

nclb¹ homozygous and nclb¹/nclb² trans-heterozygous mutant testes do not exhibit multicellular cysts beyond four to eight cells. Fewer differentiating cysts are found, indicating that cysts degenerate after the four- to eight-cell stage. There isn't an increase in apoptosis (according to activated caspase levels) in these mutants. A few large germ cells are observed further from the hub, but these only contain round fusome material that does not extend between germ cells, indicating that any germ cells that do progress to this stage are no longer part of cysts and are developing abnormally. Somatic cysts do appear to be able to differentiate in these mutants.

nclb¹ homozygous and nclb¹/nclb² trans-heterozygous mutant testes have fewer hub-proximal germ cells compared to wild-type, with a further reduction seen at days 5 and 10. These mutant germ cells still establish a male sexual identity and transition normally to become male germline stem cells. However, these germline stem cells are not properly maintained in the testis.

Ovaries from 5-day-old nclb¹/nclb² mutant adults contain ample germline and exhibit clear signs of egg chamber development; however, some germline loss is observed in 10-15 day old females. In newly eclosed females, a few mature eggs are observed, resulting in the limited fertility of these females. However, most of the egg chambers are arrested around stage 5 of development.

(Casper et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nclb²/nclb¹ is rescued by nclb^UASp.cCa/Scer\GAL4^{VP16.nanos.UTR}

(Casper et al., 2011)

nclb²/nclb¹ is rescued by nclb^UASp.cCa/Scer\GAL4^αTub84B.PL

(Casper et al., 2011)

nclb²/nclb¹ is rescued by nclb^UASp.cCa/Scer\GAL4^da.PU

(Casper et al., 2011)

Comments

Expression of nclb^{Scer\UAS.P\T.cCa} in the germline under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is sufficient to rescue the germline loss phenotype observed in nclb¹/nclb² mutants. Rescued testes have a wild-type appearance, with numerous germline stem cells associated with the hub and ample numbers of differentiating spermatogenic cysts. In addition, these rescued males have elongated spermatids and are completely fertile.

Expression of nclb^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^αTub84B.PL rescues the germline and fertility defects found in nclb¹/nclb² mutants.

(Casper et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dpwp1^nclb1

(Liu et al., 2017)

nclb¹

(Casper et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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