FlyBase Allele Report: Dmel\nclb[2]

General Information

Symbol

Dmel\nclb²

Species

D. melanogaster

Name

FlyBase ID

FBal0263562

Feature type

allele

Associated gene

Dmel\nclb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Casper et al., 2011)

Mutagen

P-element activity

(Casper et al., 2011)

Progenitor genotype

Caused by aberration

Df(2R)nclb

Cytology

Description

Imprecise excision of P{EPgy2}CG6751^EY10712 results in a 2170bp deletion that removes the entire nclb coding region and the adjacent upstream gene CG30016.

(Casper et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chamber | oogenesis stage S5

(Casper et al., 2011)

egg chamber | oogenesis stage S5 (with nclb¹)

(Casper et al., 2011)

egg chamber | oogenesis stage S5 | somatic clone

(Casper et al., 2011)

female germline cell

(Casper et al., 2011)

female germline cell (with nclb¹)

(Casper et al., 2011)

female germline cell | somatic clone

(Casper et al., 2011)

follicle cell | somatic clone

(Casper et al., 2011)

individualization stage spermatid

(Casper et al., 2011)

individualization stage spermatid (with nclb¹)

(Casper et al., 2011)

macrochaeta | adult stage (with nclb¹)

(Liu et al., 2017)

male germline cell

(Casper et al., 2011)

male germline cell (with nclb¹)

(Casper et al., 2011)

nurse cell | oogenesis stage S5

(Casper et al., 2011)

nurse cell | oogenesis stage S5 (with nclb¹)

(Casper et al., 2011)

nurse cell | oogenesis stage S5 | somatic clone

(Casper et al., 2011)

ovary

(Casper et al., 2011)

ovary (with nclb¹)

(Casper et al., 2011)

testis

(Casper et al., 2011)

testis (with nclb¹)

(Casper et al., 2011)

Detailed Description

Statement

Reference

nclb¹/nclb² transheterozygotes are semi-viable, exhibit delayed pupation, and both larvae and pupae have a significantly smaller body size/volume, as compared to controls. These individuals also show shorter and thinner bristles, as compared to controls. nclb² homozygotes die as small larvae despite of their normal food consumption, compared to controls.

(Liu et al., 2017)

nclb² homozygous mutant larvae are smaller in size than heterozygous siblings and fail to enter pupariation; in rare cases, nclb² mutant larvae live up to 9 or 10 days of age without forming pupae.

nclb² homozygous and nclb¹/nclb² trans-heterozygous mutant testes do not exhibit multicellular cysts beyond four to eight cells. Fewer differentiating cysts are found, indicating that cysts degenerate after the four- to eight-cell stage. There isn't an increase in apoptosis (according to activated caspase levels) in these mutants. A few large germ cells are observed further from the hub, but these only contain round fusome material that does not extend between germ cells, indicating that any germ cells that do progress to this stage are no longer part of cysts and are developing abnormally. Somatic cysts do appear to be able to differentiate in these mutants.

nclb² mutant germline stem cell clones are present in similar numbers to wild-type clones 2 days after clone induction. However, 10 days after clone induction, the number of testes with nclb² mutant germline stem cells is dramatically reduced relative to wild-type control clones. These mutant germline clones can form multicellular cysts, although these do not progress beyond the four- to eight-cell stage. Thus, nclb² mutant germ cells are able to enter differentiation even many days after generating null mutant germline stem cell clones.

nclb¹/nclb² mutants exhibit egg chamber arrest around stage 5 of development, when the nurse cells endoreplicate and become polyploid.

There is no difference in the number of nclb² mutant germline stem cells compared to control clones even 15 days after clone induction. nclb is not required in the germline for female germline stem cell maintenance.

nclb² mutant germline stem cell clones exhibit stage 5 arrest, after endoreplication where polyploid chromatin remains in a 'five blob' configuration.

nclb² mutant follicle cell clones contribute normally to the epithelium and are observed in older egg chambers at late timepoints; however, the overall number of follicle cell clones decreases dramatically over time.

(Casper et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nclb²/nclb¹ is rescued by nclb^UASp.cCa/Scer\GAL4^{VP16.nanos.UTR}

(Casper et al., 2011)

nclb²/nclb¹ is rescued by nclb^UASp.cCa/Scer\GAL4^αTub84B.PL

(Casper et al., 2011)

nclb²/nclb¹ is rescued by nclb^UASp.cCa/Scer\GAL4^da.PU

(Casper et al., 2011)

Comments

Expression of nclb^{Scer\UAS.P\T.cCa} in the germline under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is sufficient to rescue the germline loss phenotype observed in nclb¹/nclb² mutants. Rescued testes have a wild-type appearance, with numerous germline stem cells associated with the hub and ample numbers of differentiating spermatogenic cysts. In addition, these rescued males have elongated spermatids and are completely fertile.

Expression of nclb^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^αTub84B.PL rescues the germline and fertility defects found in nclb¹/nclb² mutants.

Expression of nclb^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^αTub84B.PL rescues the lethality found in homozygous nclb² mutants. Fertility is not rescued in these mutants.

Expression of nclb^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^αTub84B.PL fails to rescue nclb¹/nclb² mutant stage 5 egg chamber arrest and fertility defects. More general expression using Scer\GAL4^αTub84B.PL, also fails to completely rescue the stage 5 nurse endoreplication phenotype but does partially rescue fertility. Expression with Scer\GAL4^da.G32, which expresses strongly in both the germline and the soma, is sufficient to rescue both the egg chamber arrest and fertility defects of nclb¹/nclb² mutants.

(Casper et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dpwp1^nclb2

(Liu et al., 2017)

nclb²

(Casper et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (2)

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