Transgenic perM2M1 flies in a per01 mutant genetic background exhibit sleep patterns that are different to those previously described for wild-type strains at 18[o]C and 25[o]. While daytime sleep is reduced compared with wild-type, nighttime sleep appears normal. The transgenic mutant flies also sleep less during the nighttime in the presence of light at 18[o]C and 25[o]. However, at the elevated temperature of 29[o]C, daytime sleep is similar for transgenic mutant and wild-type flies, and high temperature strongly inhibits daytime activity in both perM2M1 , per01 transgenic mutants and wild-type.
per01 flies rescued with perM2M1 generally show earlier onsets of evening activity and shorter, less robust, midday siestas compared to per01 flies rescued with per8:8.