FB2026_02 , released June 18, 2026
Allele: Dmel\Ada2ad189
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General Information
Symbol
Dmel\Ada2ad189
Species
D. melanogaster
Name
FlyBase ID
FBal0264245
Feature type
allele
Associated gene
Dmel\Ada2a
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Pankotai et al., 2005)
Progenitor genotype
P{lacW}moiS096713
(Pankotai et al., 2005)
Caused by aberration
Df(3R)d189
Cytology
Description

Imprecise excision of the progenitor insertion has resulted in the removal of nucleotides +107 to -613 relative to the transcription start site of the Rpb4-Ada2a common first exon.

(Pankotai et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Df(3R)d189 homozygotes or Df(3R)d189/Df(3R)P14 animals in which the function of all genes affected by Df(3R)d189 except Ada2a have been rescued using P{CaSpeR4-Dtl+Rpb4+} are either late-larval of early-pupal lethals. The animals follow a seemingly normal course of development until the third larval instar stage, but approximately a quarter of them (27%) never pupate. Three quarters (73%) form prepupae, which are similar to larvae in shape, are covered with a brownish cuticle, and die at this stage.

      Females with Df(3R)d189/Df(3R)P14 P{CaSpeR4-Dtl+Rpb4+} germlines are not recovered in experiments transplanting mutant germline cells into host embryos, indicating that Ada2a function is required in the germline.

      In disc-derived body-parts, homozygous Ada2ad189 clones (homozygous for Df(3R)d189 with the function of all genes affected by the deficiency except Ada2a having been rescued using P{CaSpeR4-Dtl+Rpb4+}) develop as frequently as control clones, but are significantly smaller than control clones. Homozygous clones on the abdomen are as large as control clones.

      (Pankotai et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Ada2ad189 is rescued by Scer\GAL4Act.PU/Ada2aUAS.cVa

      (Vamos and Boros, 2012)
      Partially rescued by

      Ada2ad189 is partially rescued by Scer\GAL4Act.PU/Ada2a::Ada2b2bS.2a.UAS

      (Vamos and Boros, 2012)

      Ada2ad189 is partially rescued by Scer\GAL4Act.PU/Ada2a::Ada2b2bM.2a.UAS

      (Vamos and Boros, 2012)
      Not rescued by

      Ada2ad189 is not rescued by Scer\GAL4Act.PU/Ada2bUAS.cVa

      (Vamos and Boros, 2012)

      Ada2ad189 is not rescued by Ada2a::Ada2b2bL.2a.UAS/Scer\GAL4Act.PU

      (Vamos and Boros, 2012)

      Ada2ad189 is not rescued by Scer\GAL4Act.PU/Ada2a::Ada2b2a2b.UAS

      (Vamos and Boros, 2012)
      Comments

      Expression of Ada2aScer\UAS.cVa under the control of Scer\GAL4Act.PU completely rescues the late third instar larval lethality of Ada2ad189 animals.

      Expression of Ada2bScer\UAS.cVa under the control of Scer\GAL4Act.PU fails to rescue the late third instar larval lethality of Ada2ad189 animals.

      Expression of either Ada2a::Ada2b2a2b.Scer\UAS or Ada2a::Ada2b2bL.2a.Scer\UAS under the control of Scer\GAL4Act.PU fails to rescue the late third instar larval lethality of Ada2ad189 animals.

      Expression of Ada2a::Ada2b2bS.2a.Scer\UAS under the control of Scer\GAL4Act.PU partially rescues the late third instar larval lethality of Ada2ad189 animals; 19% reach the P5 pupal stage and 2% reach the P12 stage.

      Expression of Ada2a::Ada2b2bM.2a.Scer\UAS under the control of Scer\GAL4Act.PU partially rescues the late third instar larval lethality of Ada2ad189 animals; 40% reach the P5 pupal stage and 1.9% reach the P12 stage.

      (Vamos and Boros, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ada2a189
      (Grau et al., 2008)
      Ada2ad189
      dAda2ad189
      (Vamos and Boros, 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)