FlyBase Allele Report: Dmel\Mical[UAS.s]

General Information

Symbol

Dmel\Mical^UAS.s

Species

D. melanogaster

Name

FlyBase ID

FBal0264688

Feature type

allele

Associated gene

Dmel\Mical

Associated Insertion(s)

Carried in Construct

P{UAS-Mical.s}

Also Known As

UAS:Mical

Key Links

Transgenic product class

wild_type

(Hung et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hung et al., 2010)

Mutagen

in vitro construct

(Hung et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Mical.s}

Cytology

Description

UAS regulatory sequences drive expression of the full-length "short" (approximately 8.2kb) isoform of Mical.

(Hung et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mical

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act5C.PI

(Hung et al., 2013)

lethal, with Scer\GAL4^how-24B

(Hung et al., 2013)

visible, with Scer\GAL4^B11-98

(Hung et al., 2010)

Phenotype Manifest In

filamentous actin, with Scer\GAL4^B11-98

(Hung et al., 2013, Hung et al., 2010)

larval somatic muscle cell | third instar larval stage, with Scer\GAL4^how-24B

(Hung et al., 2013)

macrochaeta, with Scer\GAL4^B11-98

(Hung et al., 2013, Hung et al., 2010)

Detailed Description

Statement

Reference

Expression of Mical^Scer\UAS.s under the control of Scer\GAL4^B11-98 results in branched bristles. F-actin is abnormally organized within these bristles

Scer\GAL4^how-24B-mediated expression of Mical^Scer\UAS.s results in abnormal actin organization in larval muscles.

(Hung et al., 2013)

Expression of Mical^Scer\UAS.s under the control of Scer\GAL4^B11-98 results in branched bristles. The number of branches per mutant bristle is increased if the flies carry two copies of Mical^Scer\UAS.s instead of one.

F-actin bundles in the developing bristles of mutant adults expressing two copies of Mical^Scer\UAS.s under the control of Scer\GAL4^B11-98 are significantly thinner than normal and form a complex meshwork of short filaments.

(Hung et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mical^UAS.s, Scer\GAL4^Act5C.PI has lethal phenotype, suppressible by SelR^UAS.cHa, Scer\GAL4^Act5C.PI

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^how-24B has lethal phenotype, suppressible by SelR^UAS.cHa, Scer\GAL4^how-24B

(Hung et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, enhanceable by SelR^C124S.UAS, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, enhanceable by Df(3R)SelR^Delta3/+

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, enhanceable by Df(3R)Exel7305/+

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, enhanceable by SelR^LL01897/SelR[+]

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, enhanceable by SelR^KK108939, Scer\GAL4^B11-98

(Hung et al., 2013)

NOT Enhanced by

Statement

Reference

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, non-enhanceable by MsrA^KK109010, Scer\GAL4^B11-98

(Hung et al., 2013)

Suppressed by

Statement

Reference

Mical^UAS.s, Scer\GAL4^how-24B has larval somatic muscle cell | third instar larval stage phenotype, suppressible by SelR^UAS.cHa, Scer\GAL4^how-24B

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, suppressible by SelR^EY22443, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, suppressible by SelR^EP3340, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, suppressible by SelR^UAS.cHa, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, suppressible by SelR^UAS.EGFP, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has filamentous actin phenotype, suppressible by SelR^UAS.cHa, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98, SelR^C124S.UAS has macrochaeta phenotype, suppressible by Act5C^M44L.UAS.GFP, Scer\GAL4^B11-98

(Hung et al., 2013)

NOT suppressed by

Statement

Reference

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, non-suppressible by MsrA^EY05753, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, non-suppressible by Txl^HP35249, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, non-suppressible by Trx2^GE14911, Scer\GAL4^B11-98

(Hung et al., 2013)

Mical^UAS.s, Scer\GAL4^B11-98 has macrochaeta phenotype, non-suppressible by dhd^EY08782, Scer\GAL4^B11-98

(Hung et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of SelR^EY22443, SelR^EP3340, SelR^Scer\UAS.cHa or SelR^{Scer\UAS.T:Avic\GFP-EGFP} strongly suppresses the branched bristle phenotype seen upon Scer\GAL4^B11-98-mediated expression of Mical^Scer\UAS.s.

Co-expression of SelR^Scer\UAS.cHa suppresses the actin alterations seen within bristles upon Scer\GAL4^B11-98-mediated expression of Mical^Scer\UAS.s.

Co-expression of SelR^{C124S.Scer\UAS} enhances the branched bristle phenotype seen upon Scer\GAL4^B11-98-mediated expression of Mical^Scer\UAS.s, increasing both number and length of branches. Addition of Act5C^{M44L.Scer\UAS.T:Avic\GFP} into this background abrogates the effect of SelR^{C124S.Scer\UAS}.

Co-expression of SelR^Scer\UAS.cHa suppresses the actin organization defects seen in larval muscles of Scer\GAL4^how-24B Mical^Scer\UAS.s animals.

Df(3R)SelR^Delta3/+, Df(3R)Exel7305/+ or SelR^LL01897/+ enhances the branched bristle phenotype seen upon Scer\GAL4^B11-98-mediated expression of Mical^Scer\UAS.s.

Co-expression of SelR^KK108939 but not Eip71CD^KK109010 enhances the branched bristle phenotype seen upon Scer\GAL4^B11-98-mediated expression of Mical^Scer\UAS.s.

(Hung et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mical^Scer\UAS.s

Mical^UAS.s

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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