Homozygous larvae show posterior paralysis which is often accompanied by an upward "tail-flip" during crawling. The segmental nerves of these larvae show large accumulations of synaptic proteins.

Neuroblasts in the brains of homozygous larvae have a reduced mitotic index (decreased metaphase and prometaphase) and abnormal mitotic figures are seen. BrdU incorporation is reduced compared to controls and disorganisation of the cycling cells is seen. Neuronal apoptosis is increased 35 times compared to wild type.

Egg chambers in homozygous female germline clones consist of 16 nurse cells (the oocyte does not differentiate).

Axonal transport is disrupted in homozygous larvae; very little movement of vesicles in either direction is seen along the axons in the mutants. For the axons where vesicle movement is seen, segment velocity (the distance travelled by a particle during an unbroken run) is reduced and pause frequency (the number of pauses per μm) is increased for both anterograde and retrograde movements. The distribution of particle reversal frequency suggests a trend towards increased reversal from anterograde to retrograde movement in the mutants. Anterograde and retrograde mitochondrial movement along neurons is also reduced in the mutant larvae compared to wild type.