FlyBase Allele Report: Dmel\fra::robo1[U.UAS.Tag:MYC]

General Information

Symbol

Dmel\fra::robo1^{U.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0264795

Feature type

allele

Associated gene

fra::robo1

Associated Insertion(s)

Carried in Construct

P{UAS-fra::robo1.U}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Coleman et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-fra::robo1.U}

Cytology

Description

UAS regulatory sequences drive expression of an uncleavable form of robo1 in which the three Fn domains have been replaced with the first three Fibronectin type II (FnIII) domains of fra. The construct is tagged with Tag:MYC.

(Coleman et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

fra::robo1

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage 16, with Scer\GAL4^eg-Mz360

(Coleman et al., 2010)

Phenotype Manifest In

commissure | embryonic stage 16, with Scer\GAL4^eg-Mz360

(Coleman et al., 2010)

Detailed Description

Statement

Reference

Misexpression of robo^{Scer\UAS.T:Hsap\MYC} in the contralateral eagle (eg) neurons under the control of Scer\GAL4^eg-Mz360 prevents the posterior subset of eg axons, but not the anterior, from crossing the midline.

(Coleman et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

fra::robo1^{U.UAS.Tag:MYC}/Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of robo1^unspecified

(Coleman et al., 2010)

Scer\GAL4^ap-md544/fra::robo1^{U.UAS.Tag:MYC} is a suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of robo1^unspecified

(Coleman et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

fra::robo1^{U.UAS.Tag:MYC}/Scer\GAL4^elav.PLu is a suppressor of larval longitudinal connective | embryonic stage 16 phenotype of robo1^unspecified

(Coleman et al., 2010)

fra::robo1^{U.UAS.Tag:MYC}/Scer\GAL4^elav.PLu is a suppressor of commissure | embryonic stage 16 phenotype of robo1^unspecified

(Coleman et al., 2010)

Scer\GAL4^ap-md544/fra::robo1^{U.UAS.Tag:MYC} is a suppressor of larval longitudinal connective | embryonic stage 16 phenotype of robo1^unspecified

(Coleman et al., 2010)

Scer\GAL4^ap-md544/fra::robo1^{U.UAS.Tag:MYC} is a suppressor of commissure | embryonic stage 16 phenotype of robo1^unspecified

(Coleman et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Pan-neuronal expression of fra::robo^{U.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PLu partially suppresses the midline crossing phenotype seen in robo^unspecified mutants. Many of the most medial FasII axons still cross the midline.

Expression of fra::robo^{U.Scer\UAS.T:Hsap\MYC} in the ap neurons under the control of Scer\GAL4^ap-md544 partially suppresses the midline crossing phenotype seen in robo^unspecified mutants. In some segments the ap axon tracts on either side of the midline are fused and collapsed on the midline.

(Coleman et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

fra::robo1^{U.Scer\UAS.T:Hsap\MYC}

fra::robo1^{U.UAS.Tag:MYC}

fra::robo^{U.Scer\UAS.T:Hsap\MYC}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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