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General Information
Symbol
Dmel\Upf214J
Species
D. melanogaster
Name
FlyBase ID
FBal0264866
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A 14bp deletion which causes a frame shift at codon 562 followed by a stop 40 codon later.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Upf214J contains a 14bp deletion (1682-1695) approximately halfway through the 1,241-residue coding sequence of Upf2, which causes a frame shift at codon 562 followed by a stop 40 codon later.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

28.9% of eggs derived from homozygous female germline clones show a fused dorsal appendage phenotype. Eggs also occasionally show a "curled" phenotype, in which the ends of the dorsal appendages curl around to form a hook-like shape. None of the eggs hatch.

Homozygous follicle cell clones are smaller than wild-type clones both in early, proliferating egg chambers and in late, endocycling egg chambers.

Mutant third instar larvae can live for up to 13 days before dying. Approximately 10% of these larvae attempt to pupate, but normal head inversion fails to occur.

Upf214J mutants do not exhibit defects in larval cell differentiation. Larval tracheal cells lacking Upf2 display wild-type morphology at all levels of branching, including tracheal terminal cell clones, which show normal branching patterns and luminal structures. Likewise, type IV da neurons appear normal in these mutants.

While homozygous mutant Upf214J clones are readily obtained from the larval tracheal system, clones are not recovered in the adult system. Large clones are also not recovered from the adult epidermis or eyes, Although small peripheral eye clones are occasionally observed.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Additional Comments
Genetic Interactions
Statement
Reference

A WGMR.PG background, used to eliminate all heterozygous and wild-type cells in the developing eye, Upf214J cells proliferate, differentiate, and form an eye, although the eyes are smaller and more disorganised (rougher) than those of controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)