FlyBase Allele Report: Ecol\lexA[VP16.Gr5a.cGa]

General Information

Symbol

Ecol\lexA^{VP16.Gr5a.cGa}

Species

E. coli

Name

FlyBase ID

FBal0265027

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{Gr5a-LexA-VP16}

Also Known As

Gr5a-LexA, Gr5a-LexA::VP16

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Gordon and Scott, 2009)

Progenitor genotype

Carried in construct

P{Gr5a-LexA-VP16}

Cytology

Description

The Gr5a promoter controls expression of a lexA::VP16 driver.

(Gordon and Scott, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

Gr5a

Encoded product / tool

lexA::VP16

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

prothoracic leg taste bristle segmental tarsal chemosensory neuron

(Thoma et al., 2016)

sugar-sensing neuron of labellar taste bristle

(Gordon and Scott, 2009)

sugar-sensing neuron | subset

(Sapkal et al., 2024)

Additional Information

Statement

Reference

Drives expression in sugar-sensing neurons of the labellar taste bristles and of the prothoracic leg taste bristles.

(FlyBase Curators, 2017)

EcollexA^{VP16.Gr5a.cGa} drives expression in all sweet taste receptor neurons in the labellar taste hairs, and in about 9 segmental tarsal sugar-sensing chemosensory neurons in the prothoracic leg that project to the prothoracic ganglion.

(Thoma et al., 2016)

The dendrites of the EcollexA^{Gr5a.T:HsimVP16} sugar-sensitive neurons of gustatory sensilla and the ScerGAL4^E49 labelled motor neurons are in close proximity. However, GFP reconstitution across synaptic partners (GRASP) did not reveal any contacts between them.

(Gordon and Scott, 2009)

Marker for

Reflects expression of

Gr5a

(Thoma et al., 2016, Gordon and Scott, 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Gr61a.4.9, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

abnormal chemosensory behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Gr64f.9.7, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

abnormal locomotor behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Gr61a.4.9, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

abnormal locomotor behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Gr64f.9.7, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

abnormal memory | adult stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Gr64f.9.7, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

abnormal proboscis extension reflex | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}

(Zhou et al., 2019)

abnormal proboscis extension reflex | conditional, with Crei\ChR2^lexAop.cKa

(Mann et al., 2013)

abnormal tarsal response, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Gr64f.9.7, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus} with 617 nm (red) light) of Ecol\lexA^{VP16.Gr5a.cGa} cells in adults induces proboscis extensions, compared to controls.

(Zhou et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

93014

w¹¹¹⁸; P{Gr5a-LexA-VP16}2; TM2/TM6

93015

w¹¹¹⁸; Bl¹/CyO; P{Gr5a-LexA-VP16}3

93013

w¹¹¹⁸, P{Gr5a-LexA-VP16}1; Bl¹/CyO; TM2/TM6

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ecol\lexA^{Gr5a.T:Hsim\VP16}

Ecol\lexA^{VP16.Gr5a.cGa}

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

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