FB2021_01, released Feb 18, 2021

Allele: Scer\GAL80IVS.WPRE.8xlexAop

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General Information
Symbol
Scer\GAL80IVS.WPRE.8xlexAop
Species
S. cerevisiae
Name
FlyBase ID
FBal0247344
Feature type
allele
Associated gene
Scer\GAL80
Associated Insertion(s)
Carried in Construct
P{8XLexAop2-IVS-GAL80-WPRE}
Also Known As
LexAop-GAL80, LexAop2-GAL80, pJFRC20-8xLexAop2-IVS-GAL80-WPRE
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Pfeiffer, 2010.9.22)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{8XLexAop2-IVS-GAL80-WPRE}
      Cytology
      Nature of the lesion
      Statement
      Reference

      8 copies of the lexAop site followed by an Hsp70 basal promoter are fused upstream of GAL80. A 67bp intron derived from Mhc has been inserted in the 5' UTR. The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) has been inserted in the 3' UTR.

      (Pfeiffer, 2010.9.22)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      lexAop
      Encoded product / tool
      GAL80
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class

      abnormal chemosensitive behavior | adult stage, with Ecol\lexAVP16.Gr5a.cGa, Hsap\KCNJ2UAS.EGFP, Scer\GAL4Gr61a.4.9

      (Thoma et al., 2016)

      abnormal chemosensitive behavior | adult stage, with Ecol\lexAVP16.Gr5a.cGa, Hsap\KCNJ2UAS.EGFP, Scer\GAL4Gr64f.9.7

      (Thoma et al., 2016)

      abnormal feeding behavior | adult stage, with Ecol\lexAGMR65C11, Hsap\KCNJ2UAS.cUa.EGFP, Scer\GAL4Gr28b.b.4.7

      (Zhan et al., 2016)

      abnormal feeding behavior | adult stage | conditional, with Crei\ChR1::Vcar\ChR1::Vcar\ChR2ReaChR.UAS.Citrine, Ecol\lexAGMR65C11, Scer\GAL4Gr28b.b.4.7

      (Zhan et al., 2016)

      abnormal locomotor behavior | adult stage, with Ecol\lexAVP16.Gr5a.cGa, Hsap\KCNJ2UAS.EGFP, Scer\GAL4Gr61a.4.9

      (Thoma et al., 2016)

      abnormal locomotor behavior | adult stage, with Ecol\lexAVP16.Gr5a.cGa, Hsap\KCNJ2UAS.EGFP, Scer\GAL4Gr64f.9.7

      (Thoma et al., 2016)

      abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Ecol\lexAChAT-MI04508-TlexA:QFAD.0, Scer\GAL40273-G4

      (Stern et al., 2019)

      abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Ecol\lexAGMR61H03, Scer\GAL40273-G4

      (Stern et al., 2019)

      abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Ecol\lexAp65.Trh, Scer\GAL40273-G4

      (Stern et al., 2019)

      abnormal memory | adult stage, with Ecol\lexAGMR44E04, Scer\GAL4Mef2.247, mAChR-AJF02725

      (Bielopolski et al., 2019)

      abnormal memory | adult stage, with Ecol\lexAVP16.Gr5a.cGa, Hsap\KCNJ2UAS.EGFP, Scer\GAL4Gr64f.9.7

      (Thoma et al., 2016)

      abnormal tarsal response, with Ecol\lexAVP16.Gr5a.cGa, Hsap\KCNJ2UAS.EGFP, Scer\GAL4Gr64f.9.7

      (Thoma et al., 2016)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (5)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL80IVS.WPRE.8xEcol\lexAop
      Scer\GAL80IVS.WPRE.8xlexAop
      Name Synonyms
      Secondary FlyBase IDs
        References (16)