FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Ent23
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General Information
Symbol
Dmel\Ent23
Species
D. melanogaster
Name
FlyBase ID
FBal0265150
Feature type
allele
Associated gene
Dmel\Ent2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Knight et al., 2010)
Mutagen
P-element activity
(Knight et al., 2010)
Progenitor genotype
Ent2P124
(Knight et al., 2010)
Cytology
Description

Imprecise excision of P{lacW}Ent2P124 resulting in the removal of the first 20 bp of the 5' untranslated region (UTR) of Ent2 as well as 227 bp of the intergenic region between Ent2 and CG9596.

(Knight et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:6,562,113..6,562,372 [+]
Comment:

Approximate endpoints of deletion resulting from the imprecise excision of P{lacW}Ent2P124. The deletion is reported as removing the first 20 bases of the Ent2 5'UTR and 227 bp of the intergenic region between Ent2 and CG9596. It is not clear what coordinate was used as the transcription start site.

(Knight et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ent23 mutants show defects in olfactory based associative learning. The olfactory acuity of Ent23 mutants is unimpaired.

      Synaptic strength and plasticity at the neuromuscular junction (NMJ) are impaired in Ent23 third instar larvae. The amplitude of spontaneous miniature excitatory junction potentials (mEJPs) is not significantly different in Ent23 mutants and wild-type controls. The frequency of MEJPs, however, is significantly elevated in Ent23 mutants relative to controls. The amplitude of stimulus evoked excitatory junction potentials (EJPs, reflecting synaptic transmitter release) is significantly increased in Ent23 mutants. Paired-pulse plasticity of Ent23 mutant synapses is impaired, while post-tetanic potentiation is normal.

      Calcium influx is elevated in Ent23 mutant motor neurons.

      (Knight et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Ent23 has abnormal learning | recessive phenotype, non-enhanceable by AdoR1

      (Knight et al., 2010)
      Suppressed by
      Statement
      Reference

      Ent23 has abnormal neurophysiology | recessive | third instar larval stage phenotype, suppressible by AdoR1

      (Knight et al., 2010)
      NOT suppressed by
      Statement
      Reference

      Ent23 has viable phenotype, non-suppressible by AdoR1

      (Knight et al., 2010)

      Ent23 has abnormal learning | recessive phenotype, non-suppressible by AdoR1

      (Knight et al., 2010)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Ent23 has synapse phenotype, suppressible by AdoR1

      (Knight et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Flies homozygous for both Ent23 and AdoR1 are completely viable.

      The performance index of Ent23, AdoR1 double mutants in associative learning is not significantly different from the performance indices of either the Ent23 of AdoR1 single mutants.

      The synaptic defects observed in either Ent23 or AdoR1 single mutants are suppressed in Ent23, AdoR1 double mutants. EJP amplitudes are not significantly different in the double mutant and wild-type controls. Ent23, AdoR1 double mutants also show normal paired-pulse facilitation and calcium influx.

      (Knight et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ent23
      ent23
      (Lin et al., 2021, Poernbacher and Vincent, 2018, Knight et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)