FlyBase Allele Report: Dmel\AdoR[1]

General Information

Symbol

Dmel\AdoR¹

Species

D. melanogaster

Name

FlyBase ID

FBal0191589

Feature type

allele

Associated gene

Dmel\AdoR

Associated Insertion(s)

TI{TI}AdoR¹

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Dolezal et al., 2005)

ends-out gene targeting

(Dolezal et al., 2005)

Progenitor genotype

Associated Insertion(s)

TI{TI}AdoR¹

Cytology

Description

Stop codon after amino acid 278.

(Knight et al., 2010)

Mutation within AdoR.

(Dolezal et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | recessive

(Knight et al., 2010)

abnormal neurophysiology | recessive | third instar larval stage

(Knight et al., 2010)

abnormal oxidative stress response

(Zemanová et al., 2016)

abnormal starvation stress response

(Zemanová et al., 2016)

chemical resistant

(Kucerova et al., 2012)

viable

(Dolezal et al., 2005)

Phenotype Manifest In

embryonic/larval neuromuscular junction

(Knight et al., 2010)

motor neuron | third instar larval stage

(Knight et al., 2010)

synapse

(Knight et al., 2010)

Detailed Description

Statement

Reference

AdoR¹/AdoR¹ 5 day old males show significantly increased mortality under starvation conditions compared to controls. AdoR¹/AdoR¹ flies show significant resistance (lower mortality rates) to paraquat toxicity (20mM) compared to wild type.

(Zemanová et al., 2016)

Feeding wild-type flies with the adenosine receptor agonist CADO (2-chloroadenosine) leads to developmental delays as well as larval and pupal lethality. AdoR¹ mutants are resistant to CADO treatment.

(Kucerova et al., 2012)

Compared with wild-type, AdoR¹ mutants display reduced performance in associative learning.

Synaptic strength and plasticity at the neuromuscular junction (NMJ) are impaired in AdoR¹ third instar larvae. Excitatory junction potentials (EJPs) are significantly elevated in AdoR¹ mutants compared with wild-type. The amplitude of spontaneous miniature excitatory junction potentials (mEJPs) is not significantly different in AdoR¹ mutants and wild-type controls. Compared with wild-type, AdoR¹ mutants show a reduction in paired-pulse plasticity at short interstimulus intervals. The amplitude of stimulus induced calcium transients is elevated in AdoR¹ mutants relative to wild-type. The increased amplitude of calcium transients in AdoR¹ mutants is attributable to an increase in calcium influx rather than impaired calcium extrusion.

(Knight et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

AdoR¹ has abnormal starvation stress response phenotype, suppressible by Akh¹/Akh¹

(Zemanová et al., 2016)

NOT Enhancer of

Statement

Reference

AdoR¹ is a non-enhancer of abnormal learning | recessive phenotype of Ent2^P124

(Knight et al., 2010)

AdoR¹ is a non-enhancer of abnormal learning | recessive phenotype of Ent2³

(Knight et al., 2010)

Suppressor of

Statement

Reference

AdoR¹/AdoR¹ is a suppressor of abnormal starvation stress response phenotype of Akh¹

(Zemanová et al., 2016)

AdoR¹ is a suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of Ent2³

(Knight et al., 2010)

AdoR¹ is a suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of Ent2^P124

(Knight et al., 2010)

AdoR¹ is a suppressor | partially of abnormal developmental rate phenotype of Dp(3;3)Karel

(Dolezal et al., 2005)

AdoR¹ is a suppressor | partially of lethal phenotype of Dp(3;3)Karel

(Dolezal et al., 2005)

AdoR¹ is a suppressor | partially of melanotic mass phenotype phenotype of Dp(3;3)Karel

(Dolezal et al., 2005)

NOT Suppressor of

Statement

Reference

AdoR¹ is a non-suppressor of viable phenotype of Ent2³

(Knight et al., 2010)

AdoR¹ is a non-suppressor of abnormal learning | recessive phenotype of Ent2³

(Knight et al., 2010)

AdoR¹ is a non-suppressor of viable phenotype of Ent2^P124

(Knight et al., 2010)

AdoR¹ is a non-suppressor of abnormal learning | recessive phenotype of Ent2^P124

(Knight et al., 2010)

Other

Statement

Reference

AdoR¹, Akh¹ has abnormal oxidative stress response phenotype

(Zemanová et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

AdoR¹ is a suppressor of synapse phenotype of Ent2³

(Knight et al., 2010)

AdoR¹ is a suppressor of synapse phenotype of Ent2^P124

(Knight et al., 2010)

AdoR¹ is a suppressor | partially of melanotic mass phenotype of Dp(3;3)Karel

(Dolezal et al., 2005)

AdoR¹ is a suppressor of embryonic/larval crystal cell phenotype of Dp(3;3)Karel

(Dolezal et al., 2005)

AdoR¹ is a suppressor | partially of lamellocyte phenotype of Dp(3;3)Karel

(Dolezal et al., 2005)

NOT Suppressor of

Statement

Reference

AdoR¹ is a non-suppressor of embryonic/larval fat body phenotype of Dp(3;3)Karel

(Dolezal et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Akh¹/Akh¹;AdoR¹/AdoR¹ double mutants have similar mortality rates to wild type flies under starvation conditions. Akh¹/Akh¹;AdoR¹/AdoR¹ double mutants have significantly higher mortality rates than controls in response to 20mM paraquat (Akh¹/Akh¹ single mutants are similar to wild type and AdoR¹/AdoR¹ single mutants show resistance to paraquat).

(Zemanová et al., 2016)

The performance index of Ent2^P124, AdoR¹ double mutants in associative learning is not significantly different from the performance indices of either the Ent2^P124 of AdoR¹ single mutants.

The performance index of Ent2³, AdoR¹ double mutants in associative learning is not significantly different from the performance indices of either the Ent2³ of AdoR¹ single mutants.

The synaptic defects observed in either Ent2^P124 or AdoR¹ single mutants are suppressed in Ent2^P124, AdoR¹ double mutants. EJP amplitudes are not significantly different in the double mutant and wild-type controls. Ent2^P124, AdoR¹ double mutants also show normal paired-pulse facilitation and calcium influx.

The synaptic defects observed in either Ent2³ or AdoR¹ single mutants are suppressed in Ent2³, AdoR¹ double mutants. EJP amplitudes are not significantly different in the double mutant and wild-type controls. Ent2³, AdoR¹ double mutants also show normal paired-pulse facilitation and calcium influx.

(Knight et al., 2010)

Introduction of the AdoR¹ mutation significantly increases pupariation and the adult emerging rate in Dp(3;3)Karel mutants. Developmental delay in the double mutant larvae is much less pronounced than in the Dp(3;3)Karel single mutant, with most of the double mutant larvae pupariating within 1 day after their control siblings. The disintegration of the fat body seen in Dp(3;3)Karel larvae is not altered by introduction of AdoR¹, but the formation of melanotic tumours is significantly reduced. The total number of circulating hemocytes is not significantly changed in the double mutant larvae compared to Dp(3;3)Karel single mutants, but the number of lamellocytes is decreased and the number of crystal cells is normal.

(Dolezal et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98119

w^*; TI{TI}AdoR¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

AdoR¹

(Trombley et al., 2023, Poernbacher and Vincent, 2018, Zemanová et al., 2016, Knight et al., 2010)

DmAdoR¹

(Kucerova et al., 2012)

adoR

(Dolezal et al., 2005)

adoR¹

(Lin et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (7)

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