UASp regulatory sequences drive expression of a mutant form of hid lacking the NRE-like sequence. The construct is tagged with Tag:FLAG.
increased cell death | embryonic stage 14, with Scer\GAL4VP16.nanos.UTR
increased cell death | embryonic stage 15, with Scer\GAL4VP16.nanos.UTR
increased cell death | embryonic stage 16, with Scer\GAL4VP16.nanos.UTR
primordial germ cell | embryonic stage 14, with Scer\GAL4VP16.nanos.UTR
primordial germ cell | embryonic stage 15, with Scer\GAL4VP16.nanos.UTR
primordial germ cell | embryonic stage 16, with Scer\GAL4VP16.nanos.UTR
Significant apoptosis is observed in the pole cells of stage 14-16 embryos expressing WΔNRE.Scer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.UTR.T:Hsim\VP16.