FlyBase Allele Report: Dmel\Skp2[ex9]

General Information

Symbol

Dmel\Skp2^ex9

Species

D. melanogaster

Name

FlyBase ID

FBal0265629

Feature type

allele

Associated gene

Dmel\Skp2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Ghorbani et al., 2011)

Mutagen

P-element activity

(Ghorbani et al., 2011)

Progenitor genotype

P{EPgy2}CG1103^EY00567

(Ghorbani et al., 2011)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes 300bp of upstream sequences and 62% of the coding sequence of Skp2.

(Ghorbani et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:4,336,414..4,337,974 [-]

Comment:

Approximate boundaries of the Skp2^ex9 deletion, which resulted from the imprecise excision of P{EPgy2}CG1103^EY00567. The deletion is described as removing 300 bp upstream of the Skp2 transcription start site and removing 62% of the coding sequence. It is shown as removing the first two exons, the first intron and most of the second intron of Skp2.

(Ghorbani et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | third instar larval stage

(Ghorbani et al., 2011)

abnormal endomitotic cell cycle | third instar larval stage (with Df(3R)Exel6140)

(Ghorbani et al., 2011)

abnormal mitotic cell cycle | third instar larval stage

(Ghorbani et al., 2011)

increased cell death | third instar larval stage

(Ghorbani et al., 2011)

lethal - all die before end of P-stage

(Ghorbani et al., 2011)

some die during P-stage

(Ghorbani et al., 2011)

Phenotype Manifest In

embryonic/larval salivary gland | third instar larval stage

(Ghorbani et al., 2011)

embryonic/larval salivary gland | third instar larval stage (with Df(3R)Exel6140)

(Ghorbani et al., 2011)

eye disc | third instar larval stage

(Ghorbani et al., 2011)

larval brain | third instar larval stage

(Ghorbani et al., 2011)

wing disc | third instar larval stage

(Ghorbani et al., 2011)

Detailed Description

Statement

Reference

Homozygotes die at the larval/pupal transition with no sign of differentiation of adult structures. Homozygous larvae are smaller than normal.

The salivary glands of feeding third instar homozygous and Skp2^ex9/Df(3R)Exel6140 larvae are significantly smaller and have less nuclei undergoing DNA replication than those of controls. The ploidy of salivary gland cell nuclei is reduced in homozygous wandering third instar larvae compared to wild type.

The brains of homozygous third instar larvae are smaller than those of controls and some of the cells appear larger than normal. Polyploid cells are seen at a low frequency.

Cells posterior to the morphogenetic furrow appear normal in homozygous eye discs but cells anterior the furrow have larger nuclei than normal which appear polyploid. This phenotype is not temperature sensitive.

Flow cytometry indicates that wing discs from newly wandering homozygous third instar larvae contain a distinct population of tetraploid (8n) cells and a smaller population of cells with greater ploidy. Extensive apoptosis is seen in the homozygous wing discs.

(Ghorbani et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Skp2[+]/Skp2^ex9 is an enhancer of abnormal mitotic cell cycle | third instar larval stage phenotype of Cks85A^ex15

(Ghorbani et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

Skp2[+]/Skp2^ex9 is an enhancer of eye disc | third instar larval stage phenotype of Cks85A^ex15

(Ghorbani et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The polyploidy seen in the eye discs of homozygous Cks85A^ex15 wandering third instar larvae anterior to the morphogenetic furrow is enhanced by Skp2^ex9/+.

Cks85A^ex15, Skp2^ex9 double homozygotes display the same lethal phase and same degree of polyploidy in cells anterior to the morphogenetic furrow in the eye disc as Skp2^ex9 single mutants.

(Ghorbani et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Skp2^ex9 is rescued by Skp2^UASp.Tag:HA/Scer\GAL4^da.G32

(Ghorbani et al., 2011)

Comments

Expression of Skp2^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 fully rescues the lethality of Skp2^ex9.

Expression of Skp2^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 rescues the polyploidy seen in nuclei anterior to the morphogenetic furrow in the eye discs of Skp2^ex9 homozygous larvae.

(Ghorbani et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59000

w^*; Skp2^ex9/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Skp2^ex9

(Vasavan et al., 2024, Jetti et al., 2023, Dui et al., 2013, Ghorbani et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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