FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\cazP398L.10x.UAS.Tag:FLAG
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General Information
Symbol
Dmel\cazP398L.10x.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0265760
Feature type
allele
Associated gene
Dmel\caz
Associated Insertion(s)
Carried in Construct
P{10xUAS-caz.P398L.FLAG}
Key Links
Transgenic product class
missense_variant
(Wang et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wang et al., 2011)
Mutagen
in vitro construct
(Wang et al., 2011)
Progenitor genotype
Carried in construct
P{10xUAS-caz.P398L.FLAG}
Cytology
Description

Ten copies of UAS regulatory sequences drive expression of a P398L mutant caz cDNA fused to an N-terminal Tag:FLAG epitope tag.

(Wang et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
caz
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  amyotrophic lateral sclerosis
      modeled by caz383
      (Baldwin et al., 2016)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of cazP398L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PI leads to a decrease in axonal transport of NPY-positive vesicles (i.e. a significant decrease in anterograde transport and a significant increase in the stationary fraction) and of mitochondria (i.e. a significant decrease in anterograde and retrograde transport and the reciprocal significant increase in the stationary fraction), in third instar larval motor neurons, as compared to controls.

      The expression of cazP398L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Toll-6-D42 does not lead to any obvious defects in viability or third instar larval locomotion, but induces a significant and progressive decrease in adult locomotion, as compared to controls.

      (Baldwin et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Toll-6-D42/cazP398L.10x.UAS.Tag:FLAG rescues caz383

      (Baldwin et al., 2016)
      Partially rescues

      Scer\GAL4CCAP.PU/cazP398L.10x.UAS.Tag:FLAG partially rescues caz383

      (Baldwin et al., 2016)
      Comments

      The expression of cazP398L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PI rescues the decreased axonal transport of mitochondria, but not of (NPY-positive) vesicles, observed in caz383 homozygous third instar larval motor neurons.

      The expression of cazP398L.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Toll-6-D42 rescues the semi-lethality, the decreased third instar larval crawling capacity and the decreased adult locomotor capacity observed in caz383 homozygotes.

      (Baldwin et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      cazP398L.10x.Scer\UAS.T:Zzzz\FLAG
      cazP398L.10x.UAS.Tag:FLAG
      cazP398L
      (Baldwin et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)