FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\FUSR522G.10x.UAS.Tag:FLAG
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General Information
Symbol
Hsap\FUSR522G.10x.UAS.Tag:FLAG
Species
H. sapiens
Name
FlyBase ID
FBal0265765
Feature type
allele
Associated gene
Hsap\FUS
Associated Insertion(s)
Carried in Construct
P{10xUAS-FUS.R522G}
Key Links
Transgenic product class
missense_variant
(Wang et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wang et al., 2011)
Mutagen
in vitro construct
(Wang et al., 2011)
Progenitor genotype
Carried in construct
P{10xUAS-FUS.R522G}
Cytology
Description

Ten copies of UAS regulatory sequences drive expression of a R522G mutant Hsap\FUS cDNA fused to an N-terminal Tag:FLAG epitope tag.

(Wang et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\FUS
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Wang et al., 2011)
      FUS:p.Arg522Gly
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      FUS:p.Arg518Gly
      FUS:p.Arg521Gly
      Associated human disease model(s)
      External database links
      ClinVar: FUS_540279
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG in motor neurons via Scer\GAL4RapGAP1-OK6 does not result in neuromuscular junction (NMJ) expansion.

      Overexpression of Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG in neurons has no significant effect on locomotion.

      Overexpression of Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG has no effect on longevity.

      (Wang et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG in the nervous system under the control of Scer\GAL4elav-C155 almost completely rescues the adult viability of males hemizygous for the 'caz1' mutant chromosome (Df(1)383 onto which a transgene containing CG32576+t5.0 has been recombined to provide CG32576[+] function) - the level of rescue seen with Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG is not significantly different from that seen when a transgene containing wild-type caz is expressed using the same driver.

      Expression of Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 does not rescue the locomotor defects seen in caz1/Y adult males.

      Expression of Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 does not rescue the the median lifespan of adult caz1/Y escapers, although maximum lifespan is increased.

      (Wang et al., 2011)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\FUSR522G.10x.Scer\UAS.T:Zzzz\FLAG
      Hsap\FUSR522G.10x.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)