The migration of subpuerineurial glial cells along the segmental nerves is stalled in tgo^C8 mutants. Approximately 68% of segments exhibit glial cell migration defects (with all glial cell types equally affected).

tgo^C8 mutants exhibit a tracheal migration phenotype.

tgo^C8 mutants exhibit characteristic wandering of peripheral nerves across segmental boundaries.

tgo^A1-42 mutants show a complete collapse of the longitudinal connectives, diagnostic of defects in the CNS midline. In addition, some peripheral nerves are mispositioned. Segmental and intersegmental nerves are not closely associated when they leave the ventral nerve cord, which is indicative for defects in the exit glial cells.