FlyBase Allele Report: Dmel\SMC3[GL00518]

General Information

Symbol

Dmel\SMC3^GL00518

Species

D. melanogaster

Name

FlyBase ID

FBal0266218

Feature type

allele

Associated gene

Dmel\SMC3

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00518}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00518}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00518

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle, with Scer\GAL4^{VP16.mat.αTub67C}

(Guo et al., 2016)

abnormal cell cycle, with Scer\GAL4^{VP16.nanos.UTR}

(Guo et al., 2016)

abnormal hatching behavior, with Scer\GAL4^{VP16.mat.αTub67C}

(Guo et al., 2016)

Phenotype Manifest In

chromosome, centromeric region | adult stage | female, with Scer\GAL4^{VP16.nanos.UTR}

(Gyuricza et al., 2016)

oocyte, with Scer\GAL4^{VP16.nanos.UTR}

(Gyuricza et al., 2016)

polar body nucleus, with Scer\GAL4^{VP16.mat.αTub67C}

(Guo et al., 2016)

Detailed Description

Statement

Reference

Eggs with expression of SMC3^GL00518 driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} do not hatch. Embryos progress through syncytial divisions that become progressively aberrant and asynchronous, with suggestions of precocious chromosome segregation; eggs appear to complete meiosis, with presence of polar body chromosomes that lack centromeric cohesion in embryos.

Half of mature stage 14 oocytes with expression of SMC3^GL00518 driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} have more than one DNA mass (precocious homologue segregation and sister chromatid cohesion along centromeres is maintained); arm cohesion during meiosis is also disrupted.

(Guo et al., 2016)

Expression of SMC3^GL00518 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} induces defects in centromere clustering and sister chromatid cohesion (assessed by number of cid foci) in pachytene oocytes.

(Gyuricza et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

SMC3^GL00518, Scer\GAL4^{VP16.nanos.UTR} has abnormal cell cycle phenotype, non-enhanceable by vtd^GL00522, Scer\GAL4^{VP16.nanos.UTR}

(Guo et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of vtd^GL00522 does not enhance precocious homologue segregation seen in oocytes with expression of SMC3^GL00518 driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Guo et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36783

y¹ sc^* v¹ sev²¹; P{TRiP.GL00518}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Cap^GL00518

SMC3^GL00518

SMC3^GL518

(Guo et al., 2016)

SMC3^RNAiGL518

(Guo et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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