Open Close
General Information
Symbol
Dmel\Sema2aUAS.GFP,Tag:M(mCd8a)
Species
D. melanogaster
Name
FlyBase ID
FBal0266708
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression Sema2a which is tagged at the C-terminus with the transmembrane region of Mmus\Cd8a and with GFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Sema2aUAS.GFP,Tag:M(mCd8a) under the control of Scer\GAL4GMR83H12 leads to subtle changes in P-Fr neurons process targeting when compared to control adults.

Embryos expressing Sema2aScer\UAS.T:Avic\GFP,T:Mmus\Cd8a under the control of Scer\GAL4twi.PG display significantly increased frequency of irregularities in the structure of embryonic salivary gland (SG) lumen compared to wild-type controls. Expression controlled by the Scer\GAL4fkh.PH driver leads to significant widening of the SG.

24.9% of hemisegments show defects in the ISNb pathway (ISNb motor axons reach muscle 12 with no RP5 innervations) in embryos expressing one copy of Sema-2aScer\UAS.T:Avic\GFP,T:Mmus\Cd8a under the control of Scer\GAL4tey-5053A. The penetrance of the phenotype increases to 52.6% of hemisegments when two copies of Sema-2aScer\UAS.T:Avic\GFP,T:Mmus\Cd8a are used.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Sema-2aScer\UAS.T:Avic\GFP,T:Mmus\Cd8a
Sema2aScer\UAS.T:Avic\GFP,T:Mmus\Cd8a
Sema2aUAS.GFP,Tag:M(mCd8a)
Name Synonyms
Secondary FlyBase IDs
    References (7)