304bp of sequence encompassing the mir-124 hairpin has been deleted and replaced by a mini-w reporter which is flanked firstly by a pair of loxP sites and secondly (flanking the loxP sites) by a pair of inverted attP sites.
The phiC31\attP sites present in the cassette that replaces the mir-124 hairpin permit subsequent genetic rescue by recombinase-mediated cassette exchange (RMCE).
The phiC31\attP sites present in the cassette that replaces the mir-124 hairpin permit subsequent genetic rescue by recombinase-mediated cassette exchange (RMCE).