FlyBase Allele Report: Dmel\Wdr24[1]

General Information

Symbol

Dmel\Wdr24¹

Species

D. melanogaster

Name

FlyBase ID

FBal0267952

Feature type

allele

Associated gene

Dmel\Wdr24

Associated Insertion(s)

PBac{RB5.WH5}Wdr24¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Cai et al., 2016)

Mutagen

FLPase

(Cash and Andrews, 2012.4.12)

Progenitor genotype

PBac{WH}Wdr24^f06205

(Cash and Andrews, 2012.4.12)

PBac{RB}e00989

(Cash and Andrews, 2012.4.12)

Associated Insertion(s)

PBac{RB5.WH5}Wdr24¹

Cytology

Description

It has the R5 breakpoints 3R:25569652 ;25570975 with predicted cytology 99B10;99B10.

(Cash and Andrews, 2012.4.12)

Allele components

Component

Use(s)

Inserted element

PBac{RB5.WH5}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:29,743,930..29,745,253 [-]

Comment:

A 1.3kb deletion in Wdr24 generated from the progenitor insertions PBac{WH}Wdr24^f06205 and PBac{RB}e00989.

(Cash and Andrews, 2012.4.12)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Wdr24¹/Wdr24¹ or Wdr24¹/Df(3R)BSC547 females have small ovaries and have a 90% reduction in eggs laid per day compared to heterozygous controls. Wdr24¹/Wdr24¹ germline clones have reduced egg chamber growth (though no oocyte loss phenotype). Wdr24¹/Wdr24¹ or Wdr24¹/Df(3R)BSC547 mutants are smaller than heterozygote siblings, with a significant decrease in body weight. Wdr24¹/Wdr24¹ clones in both fat body and somatically derived follicle cells have significantly decreased nuclear size compared to heterozygous cells. Egg chambers from well fed Wdr24¹/Wdr24¹ females and Wdr24¹/Wdr24¹ larval fat bodies show activation of autophagy, with inappropriate accumulation of autolysosomes; larval fat bodies (under both fed and starved conditions) lack autolysosome acidification and do not show autophagic flux.

(Cai et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Wdr24¹ has decreased body size | recessive | adult stage phenotype, suppressible by Nprl3^RNAi.UAS.V20/Scer\GAL4^hs.PU

(Cai et al., 2016)

Wdr24¹ has decreased body size | recessive | adult stage phenotype, suppressible by Scer\GAL4^hs.PU/Nprl2^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has decreased cell growth rate phenotype, suppressible by Nprl3^RNAi.UAS.V20/Scer\GAL4^hs.PU

(Cai et al., 2016)

Wdr24¹ has decreased cell growth rate phenotype, suppressible by Scer\GAL4^hs.PU/Nprl2^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has decreased cell growth rate phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tsc1^GL00012

(Cai et al., 2016)

Wdr24¹ has female semi-sterile | recessive phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl2^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has decreased body size | recessive phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl2^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has female semi-sterile | recessive phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl3^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has decreased body size | recessive phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl3^RNAi.UAS.V20

(Cai et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Wdr24¹ has ovary phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl2^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has ovary phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl3^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has ovary phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tsc1^GL00012

(Cai et al., 2016)

NOT suppressed by

Statement

Reference

Wdr24¹ has autolysosome phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl3^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has autolysosome phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Nprl2^RNAi.UAS.V20

(Cai et al., 2016)

Wdr24¹ has autolysosome phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tsc1^GL00012

(Cai et al., 2016)

Other

Statement

Reference

Atg7^d14/Atg7^d77, Wdr24¹ has ovariole phenotype

(Cai et al., 2016)

Atg7^d14/Atg7^d77, Wdr24¹ has lysosome phenotype

(Cai et al., 2016)

Nprl3¹, Wdr24¹ has embryonic/larval fat body | third instar larval stage phenotype

(Cai et al., 2016)

Nprl3¹, Wdr24¹ has lysosome | third instar larval stage phenotype

(Cai et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Nprl2^{dsRNA.shRNA.Scer\UAS} or Nprl3^{dsRNA.shRNA.Scer\UAS} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} significantly partially suppresses the small ovaries and reduced fertility seen in Wdr24¹/Wdr24¹ females.

Expression of Nprl2^{dsRNA.shRNA.Scer\UAS} or Nprl3^{dsRNA.shRNA.Scer\UAS} driven by Scer\GAL4^hs.PU significantly partially suppresses the reduced body size (measured body weight) seen in Wdr24¹/Wdr24¹ flies.

Expression of Nprl2^{dsRNA.shRNA.Scer\UAS} or Nprl3^{dsRNA.shRNA.Scer\UAS} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the egg chamber growth defect but not the inappropriate accumulation of autolysosomes seen in Wdr24¹/Wdr24¹ flies (though autophagy activation is reduced).

Expression of Tsc1^GL00012 driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} significantly suppresses the ovary growth defect but not the inappropriate accumulation of autolysosomes seen in Wdr24¹/Wdr24¹ egg chambers.

Wdr24¹/Wdr24¹ ;Atg7^d14/Atg7^d77 double mutant ovarioles show accumulation of late endosomes/lysosomes (not autolysosomes), without activation of autophagy.

(Cai et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Wdr24¹ is rescued by Wdr24^UASp.EGFP/Scer\GAL4^Ubi.PU

(Cai et al., 2016)

Wdr24¹ is rescued by Wdr24^UASp.EGFP/Scer\GAL4^Cg.PU

(Cai et al., 2016)

Partially rescued by

Wdr24¹ is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/Wdr24^UASp.EGFP