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General Information
Symbol
Dmel\Wdr241
Species
D. melanogaster
Name
FlyBase ID
FBal0267952
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A 1.3kb deletion in Wdr24 generated from the progenitor insertions PBac{WH}Wdr24f06205 and PBac{RB}e00989.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

It has the R5 breakpoints 3R:25569652 ;25570975 with predicted cytology 99B10;99B10.

Insertion components
PBac{RB5.WH5}Wdr241
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Wdr241/Wdr241 or Wdr241/Df(3R)BSC547 females have small ovaries and have a 90% reduction in eggs laid per day compared to heterozygous controls. Wdr241/Wdr241 germline clones have reduced egg chamber growth (though no oocyte loss phenotype). Wdr241/Wdr241 or Wdr241/Df(3R)BSC547 mutants are smaller than heterozygote siblings, with a significant decrease in body weight. Wdr241/Wdr241 clones in both fat body and somatically derived follicle cells have significantly decreased nuclear size compared to heterozygous cells. Egg chambers from well fed Wdr241/Wdr241 females and Wdr241/Wdr241 larval fat bodies show activation of autophagy, with inappropriate accumulation of autolysosomes; larval fat bodies (under both fed and starved conditions) lack autolysosome acidification and do not show autophagic flux.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Nprl2dsRNA.shRNA.Scer\UAS or Nprl3dsRNA.shRNA.Scer\UAS driven by Scer\GAL4nos.UTR.T:Hsim\VP16 significantly partially suppresses the small ovaries and reduced fertility seen in Wdr241/Wdr241 females.

Expression of Nprl2dsRNA.shRNA.Scer\UAS or Nprl3dsRNA.shRNA.Scer\UAS driven by Scer\GAL4hs.PU significantly partially suppresses the reduced body size (measured body weight) seen in Wdr241/Wdr241 flies.

Expression of Nprl2dsRNA.shRNA.Scer\UAS or Nprl3dsRNA.shRNA.Scer\UAS driven by Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the egg chamber growth defect but not the inappropriate accumulation of autolysosomes seen in Wdr241/Wdr241 flies (though autophagy activation is reduced).

Expression of Tsc1GL00012 driven by Scer\GAL4nos.UTR.T:Hsim\VP16 significantly suppresses the ovary growth defect but not the inappropriate accumulation of autolysosomes seen in Wdr241/Wdr241 egg chambers.

Wdr241/Wdr241 ;Atg7d14/Atg7d77 double mutant ovarioles show accumulation of late endosomes/lysosomes (not autolysosomes), without activation of autophagy.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Wdr24Scer\UAS.P\T.T:Avic\GFP-EGFP driven by Scer\GAL4nos.UTR.T:Hsim\VP16 partially rescues ovary size and fertility in Wdr241/Wdr241 females. Expression of Wdr24Scer\UAS.P\T.T:Avic\GFP-EGFP driven by Scer\GAL4Ubi.PU rescues body weight in Wdr241/Wdr241 females. Expression of Wdr24Scer\UAS.P\T.T:Avic\GFP-EGFP driven by Scer\GAL4cg.PU rescues inappropriate accumulation of autolysosomes in Wdr241/Wdr241 larval fat bodies.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)