FlyBase Allele Report: Dmel\Gk2[RNAi.UAS]

General Information

Symbol

Dmel\Gk2^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0268041

Feature type

allele

Associated gene

Dmel\Gk2

Associated Insertion(s)

Carried in Construct

P{UAS-Gk2.RNAi}

Also Known As

dGK-IR

Key Links

Transgenic product class

RNAi_reagent

(Wightman et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wightman et al., 2012)

Mutagen

in vitro construct

(Wightman et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Gk2.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of Gk2 sequences.

(Wightman et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Gk2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of glycerol kinase deficiency

CEA

(Wightman et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive, with Scer\GAL4^c564

(Wightman et al., 2012)

melanotic mass phenotype | third instar larval stage, with Scer\GAL4^c564

(Wightman et al., 2013)

partially lethal, with Scer\GAL4^c564

(Wightman et al., 2013)

partially lethal, with Scer\GAL4^how-24B

(Wightman et al., 2013)

partially lethal, with Scer\GAL4^αTub84B.PL

(Wightman et al., 2013)

short lived | nutrition conditional

(Wightman et al., 2012)

Phenotype Manifest In

melanotic mass | third instar larval stage, with Scer\GAL4^c564

(Wightman et al., 2013)

Detailed Description

Statement

Reference

Expression of Gk2^{dsRNA.Scer\UAS} driven by Scer\GAL4^αTub84B.PL results in larval lethality ('Gk2^{dsRNA.Scer\UAS}-let') or survival to adulthood ('Gk2^{dsRNA.Scer\UAS}-sur'), depending on the insertion line. Expression of Gk2^{dsRNA.Scer\UAS}-let (but not Gk2^{dsRNA.Scer\UAS}-sur) lines driven by Scer\GAL4^c564 or Scer\GAL4^how-24B also results in larval and pupal lethality. Scer\GAL4^c564>Gk2^{dsRNA.Scer\UAS}-let third instar larvae often show melanotic masses before lethality at the pupal stage. Expression of Gk2^{dsRNA.Scer\UAS} driven by Scer\GAL4^GMR.PS or Scer\GAL4^elav-C155 does not result in any obvious external or behavioral phenotypes.

(Wightman et al., 2013)

Flies expressing Gk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 have a lifespan similar to that of wild-type controls when raised on a sucrose only food source. However, when raised on a glycerol only food source, the mutant flies have a reduced lifespan compared to wild-type flies raised under these conditions, with the lifespan being similar to that under starvation conditions. The mutant flies show an intermediate lifespan when raised on a food source containing glycerol and sucrose.

Adult males expressing Gk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 have a small but significant decrease in survivorship on a high salt diet (3.5% and 4.0%) compared to control flies.

(Wightman et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Gk2^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, suppressible by bw[+]/bw¹⁹

(Wightman et al., 2012)

NOT suppressed by

Statement

Reference

Gk2^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, non-suppressible by Gk1^UAS.Ex, Scer\GAL4^c564

(Wightman et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Gyk^Scer\UAS.Ex does not rescue the reduced survival on a glycerol only food source of flies expressing Gk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564.

The glycerol hypersensitivity of flies expressing Gk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 (seen as a reduction in survival on a glycerol only food source) is suppressed if the flies are heterozygous for bw¹⁹.

(Wightman et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Co-expression of Gk2^Scer\UAS.Ex significantly reduces the larval lethality seen with expression of Gk2^{dsRNA.Scer\UAS}-let driven by Scer\GAL4^c564.

(Wightman et al., 2013)

Co-expression of Gk^Scer\UAS.Ex rescues the reduced survival on a glycerol only food source of flies expressing Gk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564.

(Wightman et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gk2^RNAi.UAS

Gk2^{dsRNA.Scer\UAS}

Gk2^dsRNA.UAS

Gk^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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