FlyBase Allele Report: Dmel\Appl[s.UAS.Tag:HA]

General Information

Symbol

Dmel\Appl^s.UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0268079

Feature type

allele

Associated gene

Dmel\Appl

Associated Insertion(s)

Carried in Construct

P{UAS-Appl.s.HA}

Also Known As

sAPPL

Key Links

Transgenic product class

inframe_deletion

(Wentzell et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Wentzell et al., 2012)

Mutagen

in vitro construct

(Wentzell et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Appl.s.HA}

Cytology

Description

UASt regulatory sequences drive expression of the N-terminus of Appl, which includes the start codon, the signal sequence and coding sequence up to amino acid 758. The construct is tagged with Tag:HA at the C-terminal end.

(Wentzell et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Appl

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease 1

CEA

(Rieche et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Appl^s.UAS.Tag:HA, Scer\GAL4^GMR.PU is a non-enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of Bace^GD5366, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Appl^s.UAS.Tag:HA is a suppressor of abnormal neuroanatomy | adult stage phenotype of sws¹

(Wentzell et al., 2012)

Appl^s.UAS.Tag:HA/Scer\GAL4^Appl.G1a is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

Scer\GAL4^elav-C155/Appl^s.UAS.Tag:HA is a suppressor of abnormal neuroanatomy | adult stage phenotype of vap^KS67

(Wentzell et al., 2012)

NOT Suppressor of

Statement

Reference

Appl^s.UAS.Tag:HA, Scer\GAL4^GMR.PU is a non-suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Bace^GD5366, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Appl^s.UAS.Tag:HA/Scer\GAL4^Appl.G1a is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Appl^d, SNF4Aγ^loe

(Wentzell et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Appl^s.UAS.Tag:HA is a suppressor of adult brain phenotype of sws¹

(Wentzell et al., 2012)

Appl^s.UAS.Tag:HA/Scer\GAL4^Appl.G1a is a suppressor of larval brain | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

NOT Suppressor of

Statement

Reference

Appl^s.UAS.Tag:HA/Scer\GAL4^Appl.G1a is a non-suppressor of larval brain | third instar larval stage phenotype of Appl^d, SNF4Aγ^loe

(Wentzell et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Appl^{s.Scer\UAS.T:Ivir\HA1} has no effect on the lamina degeneration phenotype seen in 28 day old flies expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU.

(Bolkan et al., 2012)

Expression of Appl^{s.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a substantially suppresses the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of Appl^{s.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a fails to suppress the vacuolisation seen in 4 day old Appl^d SNF4Aγ^loe double mutant males.

Expression of Appl^{s.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 suppresses the vacuolisation seen in futsch^olk1 mutant 6 day old flies.

Expression of Appl^{s.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 suppresses the vacuolisation seen in vap^KS67 mutant 14 day old males.

Expression of Appl^{s.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 fails to suppress the vacuolisation seen in the brains of sws¹ mutant 14 day old males.

(Wentzell et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Appl^{s.Scer\UAS.T:Ivir\HA1}

Appl^s.UAS.Tag:HA

sAPPL

(Bolkan et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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