FlyBase Allele Report: Dmel\mir-11[KO.w-]

General Information

Symbol

Dmel\mir-11^KO.w-

Species

D. melanogaster

Name

FlyBase ID

FBal0268278

Feature type

allele

Associated gene

Dmel\mir-11

Associated Insertion(s)

TI{TI}mir-11^KO.w-

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

cre recombinase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2014, Ge et al., 2012)

Mutagen

cre recombinase

(Ge et al., 2012)

Progenitor genotype

mir-11^KO.w+

(Ge et al., 2012)

Associated Insertion(s)

TI{TI}mir-11^KO.w-

Cytology

Description

Knock-out of the mir-11 gene. The expression of the E2f1 host gene appears unaffected (assayed by quantitative PCR).

(Chen et al., 2014)

The loxP cassette containing the w^+mW.hs marker has been excised. A single loxP site flanked by inverted attP sites remains.

(Ge et al., 2012)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | recessive

(Chen et al., 2014)

female fertile

(Chen et al., 2014)

male fertile

(Chen et al., 2014)

partially lethal | recessive

(Chen et al., 2014)

viable

(Ge et al., 2012)

viable (with mir-11^R.w-)

(Ge et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

3-5 day old homozygotes show reduced climbing ability in a negative geotaxis assay.

(Chen et al., 2014)

The viability of homozygous mir-11^KO.w- mutants is not different from controls. Homozygous mir-11^KO.w- mutants show no significant reduction in the completion of embryogenesis compared to controls.

(Ge et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-11^KO.w- has viable phenotype, suppressible by Df(2R)mir-309-6^Δ1

(Ge et al., 2012)

mir-11^KO.w- has viable phenotype, suppressible | partially by Df(2R)mir-309-6^Δ1/Df(3L)XR38

(Ge et al., 2012)

NOT suppressed by

Statement

Reference

mir-11^KO.w-/mir-11^R.w- has viable phenotype, non-suppressible by Df(2R)mir-309-6^Δ1

(Ge et al., 2012)

mir-11^KO.w- has viable phenotype, non-suppressible by Df(2R)mir-309-6^Δ1/Df(3L)X14

(Ge et al., 2012)

mir-11^KO.w- has viable phenotype, non-suppressible by Df(3L)H99/Df(2R)mir-309-6^Δ1

(Ge et al., 2012)

Other

Statement

Reference

Df(2R)mir-309-6^Δ1, mir-11^KO.w- has abnormal neuroanatomy phenotype

(Ge et al., 2012)

Phenotype Manifest In

Other

Statement

Reference

Df(2R)mir-309-6^Δ1, mir-11^KO.w- has larval longitudinal connective phenotype

(Ge et al., 2012)

Df(2R)mir-309-6^Δ1, mir-11^KO.w- has symmetrical commissure phenotype

(Ge et al., 2012)

Df(2R)mir-309-6^Δ1, mir-11^KO.w- has larval central nervous system phenotype

(Ge et al., 2012)

Df(2R)mir-309-6^Δ1, mir-11^KO.w- has glial cell phenotype

(Ge et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The mir-11^KO.w-, Df(2R)mir-309-6^Δ1 double mutant combination show strong reduction in survival compared with the parental lines. The mir-11^KO.w-, Df(2R)mir-309-6^Δ1 combination shows strong embryonic lethality. This lethality can be rescued by mir-11^R.w-.

mir-11^KO.w-/+, Df(2R)mir-309-6^Δ1 double mutants show a modest but statistically significant decrease in survival compared with controls. The reciprocal combination (mir-11^KO.w-, Df(2R)mir-309-6^Δ1/+) does not show any significant effect on survival compared to controls.

mir-11^KO.w-, Df(2R)mir-309-6^Δ1 double mutants display embryonic central nervous system (CNS) defects. Longitudinal connectives are thin and show occasional gaps between segments, typically interrupting the nerve cord in the mid-abdominal region. Mutant commissures are also abnormal in spacing and thickness. All the mutant embryos show abnormal CNS morphology in at least one segment. On an average, two segments per embryo lacked longitudinal connectives. These defects are not observed in double mutant embryos rescued by inclusion of Df(2R)6R, and are considerably less frequent in double mutant embryos rescued by inclusion of the rescue allele mir-11^R.w-

Glial cells are irregular in shape and spacing, and in some cases are lost in the affected region of the mir-11^KO.w-, Df(2R)mir-309-6^Δ1 double mutant embryos.

Df(3L)XR38/+ partially suppresses the lethality of mir-11^KO.w-, Df(2R)mir-309-6^Δ1 double mutants.

Df(3L)X14/+ strongly suppresses the lethality of mir-11^KO.w-, Df(2R)mir-309-6^Δ1 double mutants.

Df(3L)H99/+ suppresses the embryonic CNS phenotypes and the lethality of mir-11^KO.w-, Df(2R)mir-309-6^Δ1 double mutants.

(Ge et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58890

w^*; TI{TI}mir-11^KO.w-

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

miR-11 (white^-)

(Ge et al., 2012)

miR-11^(w-)

(Ge et al., 2012)

miR-11<up>KO (w-)</up>

(Ge et al., 2012)

mir-11^KO.w-

(Niu et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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