FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Pfas1-6
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General Information
Symbol
Dmel\Pfas1-6
Species
D. melanogaster
Name
FlyBase ID
FBal0268447
Feature type
allele
Associated gene
Dmel\Pfas
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Holland et al., 2011)
Progenitor genotype
P{EPgy2}PfasEY05576
(Holland et al., 2011)
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in an 856bp deletion starting from the insertion site and extending into the ade2 coding region (removing the predicted transcription start sites and 393bp of coding sequence). 33bp of P-element inverted repeat remain at the insertion site.

(Holland et al., 2011)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2L:6,045,125..6,045,981 [-]
Comment:

856 bp deletion resulting from the imprecise excision of P{EPgy2}PfasEY05576 extends into Pfas from the insertion site and removes 393 bp of coding sequence. 33 bp of the P-element inverted repeat remain at the site of the insertion.

(Holland et al., 2011)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutants die as predominantly at pupal stage P5, with necrotic tissue present in most of the pupae.

      Wing discs from homozygous white prepupae contain apoptotic cells.

      (Holland et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Pfas1-6 has melanotic necrosis | pupal stage P5 phenotype, non-suppressible by p535A-1-4/p53[+]

      (Holland et al., 2011)

      Pfas1-6 has melanotic necrosis | pupal stage P5 phenotype, non-suppressible by Df(3L)H99/+

      (Holland et al., 2011)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The addition of p535A-1-4/+ or Df(3L)H99/+ does not suppress the necrosis seen in stage P5 ade21-6 pupae.

      (Holland et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference

      BacA\p35Scer\UAS.cHa (in the absence of a Scer\GAL4 driver) partially suppresses the lethality and necrotic phenotypes of ade21-6 animals, with suppression being dose-dependent.

      (Holland et al., 2011)
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pfas1-6
      ade21-6
      (Holland et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)