FlyBase Allele Report: Dmel\mgr[l4]

General Information

Symbol

Dmel\mgr^l4

Species

D. melanogaster

Name

FlyBase ID

FBal0268992

Feature type

allele

Associated gene

Dmel\mgr

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Delgehyr et al., 2012)

Mutagen

Progenitor genotype

Cytology

Description

A deletion in the 5' UTR of mgr.

(Delgehyr et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle (with mgr⁵)

(Delgehyr et al., 2012)

abnormal mitotic cell cycle (with mgr⁵)

(Delgehyr et al., 2012)

lethal | pharate adult stage (with mgr⁵)

(Delgehyr et al., 2012)

partially lethal - majority die | pharate adult stage (with mgr⁵)

(Delgehyr et al., 2012)

sterile (with mgr⁵)

(Delgehyr et al., 2012)

Phenotype Manifest In

larval neuroblast (with mgr⁵)

(Delgehyr et al., 2012)

mature primary spermatocyte cyst cell (with mgr⁵)

(Delgehyr et al., 2012)

meiotic spindle (with mgr⁵)

(Delgehyr et al., 2012)

microtubule (with mgr⁵)

(Delgehyr et al., 2012)

mitotic spindle (with mgr⁵)

(Delgehyr et al., 2012)

Detailed Description

Statement

Reference

mgr^l4/mgr⁵ neuroblasts from third instar larval brains exhibit circular mitotic figures; spindles may be monopolar or bipolar with abnormal morphology. Their mitotic index and the ratio of metaphase:anaphase figures is elevated some twofold over that of wild type, and they show increased defective and monopolar mitoses. These data indicate a delay in progression through the mitotic cycle. (mgr^l4 in combination with an unspecified deficiency results in similar, but more severe, phenotypes.)

mgr^l4/mgr⁵ animals show pharate lethality with some sterile adults.

mgr^l4/mgr⁵ testes cells can undergo the premeiotic mitosis. However, meiotic spindles are either absent or highly abnormal in mature (but not young) primary spermatocyte cysts.

mgr^l4/mgr⁵ larval brain squashes show a slight loss of microtubules.

(Delgehyr et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

mgr^l4/mgr⁵ has microtubule phenotype, enhanceable by βTub56D^GFP

(Delgehyr et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Addition of βTub56D^T:Avic\GFP to the mgr^l4/mgr⁵ background results in a dramatic loss of microtubules in larval brain squashes.

(Delgehyr et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mgr^l4/mgr¹ is rescued by Scer\GAL4^Act.PU/mgr^UAS.cDa

(Delgehyr et al., 2012)

Comments

The pharate lethality associated with mgr^l4/mgr¹ can be rescued by Scer\GAL4^Act.PU-mediated expression of mgr^Scer\UAS.cDa.

(Delgehyr et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mgr^l4

(Palumbo et al., 2015, Delgehyr et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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