FlyBase Allele Report: Dmel\Arp3[83F]

General Information

Symbol

Dmel\Arp3^83F

Species

D. melanogaster

Name

FlyBase ID

FBal0269074

Feature type

allele

Associated gene

Dmel\Arp3

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: C232A.

(Rajan et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,114,640..8,114,640 [-]

Nucleotide change:

T8114640C

Amino acid change:

C232R | Arp3-PA; C232R | Arp3-PB

Reported amino acid change:

C232R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Rajan et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Rajan et al., 2009)

lethal (with Arp3^EP3640)

(Rajan et al., 2009)

Phenotype Manifest In

external sensory organ precursor cell IIa | somatic clone

(Rajan et al., 2009)

external sensory organ precursor cell IIb | somatic clone

(Rajan et al., 2009)

macrochaeta | somatic clone

(Rajan et al., 2009)

microchaeta | somatic clone

(Rajan et al., 2009)

Detailed Description

Statement

Reference

Arp3^83F clones in the adult thorax show loss of the external structures (socket and shaft cells) of the microchaetae, while macrochaetae sometimes show a double-shaft phenotype.

Within Arp3^83F pIIa-pIIb cells, an apical actin-rich structure (ARS) is formed as in wild type, but its apical area is markedly reduced and, in about 50% of cases, the stalk of the normal 'umbrella shape' is not formed properly.

(Rajan et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^sca-109-68-mediated expression of N^ECN.Scer\UAS in Arp3^83F clones, as in a wild type background, results in a multiple socket phenotype.

(Rajan et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Arp3^83F is rescued by Scer\GAL4^αTub84B.PL/Arp3^UASp.GFP

(Rajan et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

39726

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}80B Arp3^83F/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Arp3^83F

(Leibfried et al., 2013, Rajan et al., 2009)

Arp66B^83F

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close