FlyBase Allele Report: Dmel\Arp3[EP3640]

General Information

Symbol

Dmel\Arp3^EP3640

Species

D. melanogaster

Name

FlyBase ID

FBal0131796

Feature type

allele

Associated gene

Dmel\Arp3

Associated Insertion(s)

P{EP}Arp3^EP3640

Carried in Construct

Also Known As

arp66B^EP3640, Arp3^EP(3)3640, EP(3)3640

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Hudson and Cooley, 2002, Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}Arp3^EP3640

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,115,864..8,115,864 [-]

(Nahm et al., 2010, Rajan et al., 2009, Berger et al., 2008, Johnson et al., 2008, Hudson and Cooley, 2002, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Arp3^83F)

(Rajan et al., 2009)

lethal (with Arp3^515FC)

(Rajan et al., 2009)

lethal (with Arp3^1066PC)

(Rajan et al., 2009)

lethal - all die before end of P-stage | recessive

(Hudson and Cooley, 2002)

some die during pharate adult stage | recessive

(Hudson and Cooley, 2002)

Phenotype Manifest In

anterior dorsocentral bristle | pharate adult stage

(Hudson and Cooley, 2002)

embryonic myoblast

(Richardson et al., 2007)

filopodium

(Gonçalves-Pimentel et al., 2011)

larval somatic muscle cell (with Arp3^schwachling)

(Berger et al., 2008)

larval ventral nerve cord commissure (with Df(3L)pbl-X1)

(Zallen et al., 2002)

macrochaeta | pharate adult stage

(Hudson and Cooley, 2002)

myoblast | embryonic stage (with Arp3^schwachling)

(Berger et al., 2008)

nurse cell & actin filament | germ-line clone

(Hudson and Cooley, 2002)

nurse cell | germline clone

(Hudson and Cooley, 2002)

nurse cell ring canal | germline clone

(Hudson and Cooley, 2002)

oocyte | germline clone

(Hudson and Cooley, 2002)

Detailed Description

Statement

Reference

Arp3^EP3640/+ mutants embryos show no defects in development or survival.

(Sarpal et al., 2012)

Cultured primary neurons derived from mutant embryos show a significant reduction in the number of filopodia compared to control neurons.

(Gonçalves-Pimentel et al., 2011)

Arp66B^EP3640 heterozygous neuromuscular junctions do not exhibit any morphological changes.

(Nahm et al., 2010)

Homozygous Arp66B^EP3640 mutant embryos do not exhibit a strong myoblast-fusion defect.

Trans-heterozygous Arp66B^EP3640/Arp66B^schwachling exhibit a clear myoblast-fusion defect.

(Berger et al., 2008)

Arp66B^EP3640 mutants reveal a moderate myoblast fusion defect. Arp66B^EP3640 mutants show a dramatic increase in the size and number of actin foci.

(Richardson et al., 2007)

Germ-line clones of Arp66B^EP3640 lead to defects in nurse cell dumping: late stage oocytes are abnormally small, while late stage nurse cells are abnormally large. By stage 10B the arrangement of actin filament bundles in the cytoplasm of mutant nurse cells is abnormally uneven, and the diameter and shape of ring canals between these cells is severely compromised. There are approximately twice as many longitudinal ridges on anterior dorso-central bristles in Arp66B^EP3640 homozygous pharate adults, as in wild-type. A similar phenotype is seen in all other macrochaete.

(Hudson and Cooley, 2002)

Arp66B^EP3640/Df(3L)pbl-X1 embryos have breaks in ventral nerve cord commissures.

(Zallen et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Arp3^EP3640 has abnormal neuroanatomy phenotype, enhanceable by Cip4¹/Cip4[+]

(Nahm et al., 2010)

Enhancer of

Statement

Reference

Arp3^EP3640/Arp66B[+] is an enhancer of abnormal neuroanatomy phenotype of Cip4¹

(Nahm et al., 2010)

Suppressor of

Statement

Reference

Arp3^EP3640/Arp3[+] is a suppressor | partially of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Arp3^EP3640 is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Arp3^EP3640 has NMJ bouton phenotype, enhanceable by Cip4¹/Cip4[+]

(Nahm et al., 2010)

Arp3^EP3640 has neuromuscular junction phenotype, enhanceable by Cip4¹/Cip4[+]

(Nahm et al., 2010)

Arp3^EP3640 has dorsal acute muscle cell | embryonic stage phenotype, enhanceable by WASp^3D3-035

(Berger et al., 2008)

Arp3^EP3640 has myoblast | embryonic stage phenotype, enhanceable by WASp^3D3-035

(Berger et al., 2008)

Enhancer of

Statement

Reference

Arp3^EP3640/Arp66B[+] is an enhancer of NMJ bouton phenotype of Cip4¹

(Nahm et al., 2010)

Arp3^EP3640/Arp66B[+] is an enhancer of neuromuscular junction phenotype of Cip4¹

(Nahm et al., 2010)

Arp3^EP3640 is an enhancer of dorsal acute muscle cell | embryonic stage phenotype of WASp^3D3-035

(Berger et al., 2008)

Arp3^EP3640 is an enhancer of myoblast | embryonic stage phenotype of WASp^3D3-035

(Berger et al., 2008)

Suppressor of

Statement

Reference

Arp3^EP3640/Arp3[+] is a suppressor | partially of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Arp3^EP3640/Arp3[+] is a suppressor | partially of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Arp3^EP3640/Arp3[+] is a suppressor | partially of embryonic/larval cuticle | embryonic stage phenotype of α-Cat¹

(Sarpal et al., 2012)

Arp3^EP3640/Arp3[+] is a suppressor of embryonic/larval cuticle | embryonic stage phenotype of arm^043A01

(Sarpal et al., 2012)

Arp3^EP3640/Arp3[+] is a suppressor of embryonic/larval cuticle | embryonic stage phenotype of arm³

(Sarpal et al., 2012)

Arp3^EP3640 is a suppressor of primary pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Arp3^EP3640 is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Arp3^EP3640 is a suppressor of pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Other

Statement

Reference

Arp3^EP3640, DAAM[+]/DAAM^Ex68 has larval central nervous system phenotype

(Gonçalves-Pimentel et al., 2011)

Arp3^EP3640/Arp66B[+], DAAM^Ex68 has larval central nervous system phenotype

(Gonçalves-Pimentel et al., 2011)

Arp3^EP3640, Arpc1^Q25sd/Df(2L)b84a9 has larval ventral nerve cord commissure phenotype

(Zallen et al., 2002)

Arp3^EP3640/Arp66B[+], Arpc1^Q25sd/Df(2L)b84a9 has larval ventral nerve cord commissure phenotype

(Zallen et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Arp3^EP3640/+ partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

Arp3^EP3640/+ does not lead to any dorsal closure defects in embryos expressing crb^+tfos in a crb^GX24w-/crb^11A22 background.

(Flores-Benitez and Knust, 2015)

One copy of Arp3^EP3640 partially suppresses the cuticle defects seen in α-Cat¹ mutant embryos. Embryos show less severe defects on average and a fraction of embryos have normal heads.

One copy of Arp3^EP3640 partially suppresses the cuticle defects seen in arm³ mutant embryos.

One copy of Arp3^EP3640 partially suppresses the cuticle defects seen in arm^043A01 mutant embryos.

(Sarpal et al., 2012)

Arp66B^EP3640/Cip4¹ double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

(Nahm et al., 2010)

Myoblast fusion does not occur in DA1 muscles in Arp66B^EP3640, WASp^3D3-035 double mutants.

(Berger et al., 2008)

Ectopic expression of Arp66B^EP3640 suppresses the patterning defects found in Scer\GAL4^GMR.PF>cindr^{dsRNA.PC.PD.Scer\UAS} mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

(Johnson et al., 2008)

In Sop2^Q25sd/Df(2L)b84a9; Arp66B^EP3640/+ embryos, commissural axons are severely reduced.

(Zallen et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Arp3^schwachling

(Berger et al., 2008)

Rescued by

Arp3^EP3640 is rescued by Arp3^EP3640/Scer\GAL4^unspecified

(Hudson and Cooley, 2002)

Rescues

Arp3^EP3640/Scer\GAL4^unspecified rescues Arp3^EP3640

(Hudson and Cooley, 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17149

w¹¹¹⁸; P{EP}Arp3^EP3640/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Arp3³⁶⁴⁰

(Tsarouhas et al., 2019)

Arp3^EP(3)3640

(Sarpal et al., 2012, Zallen et al., 2002)

Arp3^EP3640

(Flores-Benitez and Knust, 2015, Richardson et al., 2007)

Arp66B^EP3640

(Gonçalves-Pimentel et al., 2011, Berger et al., 2008)

EP(3)3640

(Hudson and Cooley, 2002)

arp3^EP3640

(Nahm et al., 2010)

arp66B^EP3640

(Johnson et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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