FlyBase Allele Report: Dmel\Arp3[schwachling]

General Information

Symbol

Dmel\Arp3^schwachling

Species

D. melanogaster

Name

FlyBase ID

FBal0247673

Feature type

allele

Associated gene

Dmel\Arp3

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Berger et al., 2008)

Progenitor genotype

Cytology

Description

Arp66B^schwachling flies exhibit a G to A nucleotide transition at the splice acceptor site of the first intron. This point mutation possibly leads to an aberrantly spliced transcript, retaining the first intron, that becomes translated into a truncated protein of 15 amino acids.

(Berger et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,115,589..8,115,589 [-]

Nucleotide change:

G8115589A

Comment:

G to A nucleotide transition at the splice acceptor site of the first intron.

(Berger et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant stage 16 embryos show a severe myoblast fusion defect in the dorsal pharyngeal muscle. Fusion competent myoblasts show a significant decrease in the volume of F-actin foci and a corresponding increase in the frequency of filopodia emanating from an F-actin focus compared to wild type.

(Haralalka et al., 2014)

Longitudinal muscle development is not strongly disturbed in Arp3^schwachling homozygote embryos, although fewer nuclei are observed in the longitudinal muscles at stage 13, the muscle and gut morphology appears normal at stage 16.

(Rudolf et al., 2014)

Homozygous Arp66B^schwachling and Arp66B^schwachling/Df(3L)ZP1 trans-heterozygous mutants display a severe defect in myoblast fusion.

The determination of founder cells and fusion-competent myoblasts in Arp66B^schwachling mutant embryos occurs normally. The DA1 muscles in Arp66B^schwachling embryos are formed to contain, on average, three nuclei, indicating that the first fusion step, forming a bi- to trinucleated precursor cell, has completed.

Ultrathin sections of Arp66B^schwachling embryos reveal the formation of fusion pores between growing muotubes and fusion-competent myoblasts. However, no plasma membrane remnants are found between the cells, as seen in wild-type cells, indicating that Arp66B^schwachling embryos stop fusion after membrane breakdown, when a fusion pore has been formed.

Trans-heterozygous Arp66B^EP3640/Arp66B^schwachling exhibit a clear myoblast-fusion defect.

(Berger et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Arp3^schwachling has dorsal acute muscle cell | embryonic stage phenotype, enhanceable by WASp^3D3-035

(Berger et al., 2008)

Arp3^schwachling has myoblast | embryonic stage phenotype, enhanceable by WASp^3D3-035

(Berger et al., 2008)

Arp3^schwachling has dorsal acute muscle cell | embryonic stage phenotype, enhanceable by WASp³

(Berger et al., 2008)

Arp3^schwachling has myoblast | embryonic stage phenotype, enhanceable by WASp³

(Berger et al., 2008)

Enhancer of

Statement

Reference

Arp3^schwachling is an enhancer of dorsal acute muscle cell | embryonic stage phenotype of WASp^3D3-035

(Berger et al., 2008)

Arp3^schwachling is an enhancer of myoblast | embryonic stage phenotype of WASp^3D3-035

(Berger et al., 2008)

Other

Statement

Reference

Arp3^schwachling, WASp^3D3-035 has embryonic myoblast | embryonic stage 16 phenotype

(Rudolf et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Aberrant migration of the longitudinal visceral muscle founder cells is observed in stage 13 WASp^3D3-035;Arp3^schwachling double homozygote embryos and many unfused somatic myoblasts are seen at stage 16 but both the longitudinal visceral muscles and gut morphology appear normal.

(Rudolf et al., 2014)

Arp66B^schwachling WASp^3D3-035 double mutants exhibit normal visceral mesoderm development, resulting in normal gut development.

(Eriksson et al., 2010)

Myoblast fusion does not occur in DA1 muscles in Arp66B^schwachling, WASp³ double mutants.

The DA1 muscle in Arp66B^schwachling WASp^3D3-035 double mutants is mainly mononucleated, indicating that myoblasts fail to fuse completely.

The myoblast-fusion phenotype found in WASp^3D3-035 or Arp66B^schwachling single mutant embryos is strongly enhanced in the double mutant. Analysis of the DA1 muscle in the double mutant reveals only one nucleus per hemisegment, indicating that fusion is completely blocked in the double mutant.

(Berger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Arp3^EP3640

(Berger et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: pnt^3D2-026.

(Berger et al., 2008)

Separated from pnt^3D2-026 by meiotic recombination.

(Berger et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Arp3^Schwachling

(Hamp et al., 2016)

Arp3^schw

(Haralalka et al., 2014)

Arp3^schwachling

(Haralalka et al., 2014, Berger et al., 2008)

Arp66B^schwachling

(Eriksson et al., 2010)

arp3^Schwachling

(Hornbruch-Freitag et al., 2011)

arp3^schwachling

(Rudolf et al., 2014)

schwachling

(Berger et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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