FlyBase Allele Report: Dmel\Abi[Δ20]

Abi^Δ20 mutant clones in the follicular epithelium completely lack actin-rich cellular protrusions, and show strong disorganization of basal actin bundles, in contrast to wild type controls.

Females expressing Abi^{ΔWIRS.Scer\UAS} under the control of Scer\GAL4^da.PU in a Abi^Δ20 background lay eggs with shape defects, with most eggs showing normal shape, but a significant proportion exhibiting a spherical shape, in contrast to controls. Egg chambers in these females show strong defects in the planar polarized basal follicle cell actin cytoskeleton, with actin filaments no longer aligned perpendicular to the A-P axis, in contrast to controls; and follicle cells fail to migrate and show a loss of whip-like protrusions and loss of polarization of filopodial protrusions, in contrast to wild type.

(Squarr et al., 2016)

Egg chambers from female flies expressing Abi^{Scer\UAS.P\T.AW} under the control of Scer\GAL4^da.PU in a Abi^Δ20 mutant background are small and aberrantly shaped compared to controls. Some spherical but larger egg chambers are also seen. The cortical actin cytoskeleton in nurse cells is substantially reduced, resulting in the formation of multinucleated nurse cells. Free-floating ring canals are often observed in such nurse cells. Even more severe defects are seen in the actin cytoskeleton of older stage egg chambers (stage 10B). The actin bundles are severely disorganised compared to control egg chambers.

(Chen et al., 2014)

Abi^Δ20 mutant macrophages have a 'spiky' morphology.

Abi^Δ20 mutant flies exhibit highly abnormal photoreceptor axon targeting and axonal bundling.

Flies expressing Abi^Scer\UAS.cSa under the control of either Scer\GAL4^da.PU alone or Scer\GAL4^da.PU and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} in a Abi^Δ20 mutant background results in egg morphology defects.

Flies expressing Abi^Scer\UAS.cSa under the control of Scer\GAL4^da.PU in a Abi^Δ20 mutant background show disruption to the integrity of cortical actin in nurse cells and the flies are completely sterile.

The egg laid by flies expressing Abi^{Scer\UAS.ΔEna} under the control of Scer\GAL4^da.PU in a Abi^Δ20 mutant background are abnormally round compared to controls.

Flies expressing Abi^{Scer\UAS.P\T.ΔEna} under the control of Scer\GAL4^da.PU in a Abi^Δ20 mutant background show disruption to the integrity of cortical actin in nurse cells, resulting in detached cytoplasmic actin bundles and ring canals.

(Chen et al., 2014)

Homozygous Abi^Δ20 mutants show early pupal lethality.

The axons of photoreceptor R-cells of Abi^Δ20 mutants display abnormal projection patterns in the larval visual system. The R-cell axons of the mutants terminate in the brain, but the projection pattern appears highly irregular. Individual axons fasciculate and form abnormal bundles and gaps in the lamina. In the medulla, axons do not appear as individual axons anymore, but form bundles. Furthermore, large numbers of R2-R5 axons overshoot the lamina and mis-project into the medulla. The differentiation of Abi^Δ20 mutant R-cells appears normal, as the numbers of different subsets of R-cell nuclei in the mutant eye imaginal disks are indistinguishable from wild-type

The projections of Scer\FLP1^ey.3.5.hs-induced Abi^Δ20 mutant R-cells into wild-type target are largely indistinguishable from control clones.

Compared with control clones, strong axonal defects can be observed in brains within large Abi^Δ20 mutant clones.

(Stephan et al., 2011)

External Data

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Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Abi^Δ20 has abnormal neuroanatomy | recessive | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/SCAR^UASp.cZa

(Stephan et al., 2011)

Abi^Δ20 has abnormal neuroanatomy | recessive | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/SCAR^ΔB.ΔP.UAS

(Stephan et al., 2011)

Abi^Δ20 has abnormal neuroanatomy | recessive | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/SCAR^{UAS.Tag:Myr(Src64B)}

(Stephan et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Abi^Δ20 has tract | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/SCAR^UASp.cZa

(Stephan et al., 2011)

Abi^Δ20 has tract | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/SCAR^ΔB.ΔP.UAS

(Stephan et al., 2011)

Abi^Δ20 has tract | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/SCAR^{UAS.Tag:Myr(Src64B)}

(Stephan et al., 2011)

Abi^Δ20 has medulla | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/SCAR^{UAS.Tag:Myr(Src64B)}

(Stephan et al., 2011)

Enhancer of

Statement

Reference

Abi[+]/Abi^Δ20 is an enhancer | maternal effect of egg | maternal effect phenotype of Lar^5.5/Lar^13.2

(Squarr et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Abi^Δ20/+ strongly enhances the rounded egg phenotype of Lar^5.5/Lar^13.2 mutants.

(Squarr et al., 2016)

Pan-neuronal expression of SCAR^{Scer\UAS.P\T.cZa} under the control of Scer\GAL4^elav-C155 weakly suppresses the Abi^Δ20-dependent deficits in axonal targeting.

Pan-neuronal expression of SCAR^{ΔB.ΔP.Scer\UAS} under the control of Scer\GAL4^elav-C155 weakly suppresses the Abi^Δ20-dependent deficits in axonal targeting.

Pan-neuronal expression of SCAR^{Scer\UAS.T:Myr-Src64B} under the control of Scer\GAL4^elav-C155 substantially rescues the frequency and severity of the Abi^Δ20 mutant axonal targeting defects. SCAR^{Scer\UAS.T:Myr-Src64B} significantly suppresses the appearance of axonal bundles in the medulla.

(Stephan et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Abi^Δ20 is rescued by Scer\GAL4^elav-C155/Abi^UAS.cSa

(Chen et al., 2014)

Abi^Δ20 is rescued by Abi^UAS.cSa/Scer\GAL4^da.PU

(Chen et al., 2014)

Abi^Δ20 is rescued by Scer\GAL4^da.PU/Scer\GAL4^{VP16.mat.αTub67C}/Abi^UASp.cCa

(Chen et al., 2014)

Abi^Δ20 is rescued by Scer\GAL4^elav-C155/Abi^{UAS.ΔEna.ΔSH3}

(Chen et al., 2014)

Abi^Δ20 is rescued by Scer\GAL4^da.PU/Scer\GAL4^{VP16.mat.αTub67C}/Abi^{UASp.ΔEna.ΔSH3}

(Chen et al., 2014)

Abi^Δ20 is rescued by Scer\GAL4^elav-C155/Abi^UAS.cSa

(Stephan et al., 2011)

Abi^Δ20 is rescued by Abi^UAS.cSa/Scer\GAL4^da.G32

(Stephan et al., 2011)

Abi^Δ20 is rescued by Scer\GAL4^elav-C155/Abi^ΔC.UAS

(Stephan et al., 2011)

Abi^Δ20 is rescued by Scer\GAL4^da.G32/Abi^WAB-HHR.UAS

(Stephan et al., 2011)

Abi^Δ20 is rescued by Scer\GAL4^elav-C155/Abi^WAB-HHR.UAS

(Stephan et al., 2011)

Partially rescued by

Abi^Δ20 is partially rescued by Scer\GAL4^da.PU/Abi^UASp.cCa

(Chen et al., 2014)

Abi^Δ20 is partially rescued by Abi^UAS.ΔEna/Scer\GAL4^da.PU

(Chen et al., 2014)

Not rescued by

Abi^Δ20 is not rescued by Abi^UASp.AW/Scer\GAL4^da.PU

(Chen et al., 2014)

Abi^Δ20 is not rescued by Scer\GAL4^elav-C155/Abi^UAS.ΔEna

(Chen et al., 2014)

Abi^Δ20 is not rescued by Scer\GAL4^da.PU/Abi^UASp.ΔEna/Scer\GAL4^{VP16.mat.αTub67C}

(Chen et al., 2014)

Abi^Δ20 is not rescued by Abi^UAS.cSa/Scer\GAL4^GMR.PU

(Stephan et al., 2011)

Abi^Δ20 is not rescued by Abi^UAS.cSa/Scer\GAL4^repo

(Stephan et al., 2011)

Abi^Δ20 is not rescued by Scer\GAL4^elav-C155/Abi^ΔN.UAS

(Stephan et al., 2011)

Comments

Expression of Abi^{Scer\UAS.P\T.AW} under the control of Scer\GAL4^da.PU does not fully rescue the dumping defects in eggs from Abi^Δ20 mutant females. The resulting flies are almost completely sterile.

Expression of Abi^{Scer\UAS.P\T.cCa} partially rescues the dumping defects in eggs from Abi^Δ20 mutant females. The resulting flies are partially sterile.

(Chen et al., 2014)

Expression of Abi^Scer\UAS.cSa under the control of Scer\GAL4^da.PU substantially rescues the morphology defects seen in Abi^Δ20 mutant macrophages. The rescued cells exhibit stable and periodic membrane protrusions. The defects in photoreceptor axon targeting are rescued when Abi^Scer\UAS.cSa is expressed under the control of Scer\GAL4^elav-C155.

Expression of Abi^{Scer\UAS.ΔEna} under the control of Scer\GAL4^da.PU does not fully rescue the morphology defects seen in Abi^Δ20 mutant macrophages. The resulting macrophages are still less circular than controls (more spiky) and have a reduced rate of membrane protrusion. Expression of Abi^{Scer\UAS.ΔEna} under the control of Scer\GAL4^elav-C155 does not rescue the axon targeting defects seen in the Abi^Δ20 mutant optic lobe.

Expression of Abi^{Scer\UAS.ΔEna.ΔSH3} under the control of Scer\GAL4^elav-C155 rescues the photoreceptor axon targeting defects seen in the Abi^Δ20 mutant optic lobe.

Expression of Abi^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^da.PU and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues the egg morphology defects and female sterility seen in Abi^Δ20 mutant females.

Expression of Abi^{Scer\UAS.P\T.ΔEna} under the control of Scer\GAL4^da.PU and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} does not rescue the egg morphology defects and female sterility seen in Abi^Δ20 mutant females. The flies are completely sterile and the resulting eggs are smaller and abnormally round compared to controls.

Expression of Abi^{Scer\UAS.P\T.ΔEna.ΔSH3} under the control of Scer\GAL4^da.PU and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues the egg morphology defects and female sterility seen in Abi^Δ20 mutant females.

(Chen et al., 2014)

Ubiquitous expression of Abi^Scer\UAS.cSa under the control of Scer\GAL4^da.G32 rescues the lethality of the Abi^Δ20 allele.

Expression of Abi^Scer\UAS.cSa in the glial cells of Abi^Δ20 mutants under the control of Scer\GAL4^repo does not modulate the axon-bundle targeting defects in the larval visual system.

Pan-neuronal expression of Abi^Scer\UAS.cSa under the control of Scer\GAL4^elav-C155 in Abi^Δ20 mutants completely restores the axonal projection pattern.

Expression of Abi^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU does not rescue the axonal targeting defects in Abi^Δ20 mutants.

Ubiquitous expression of Abi^{WAB-HHR.Scer\UAS} under the control of Scer\GAL4^da.G32 completely rescues the lethality of Abi^Δ20 mutants.

(Stephan et al., 2011)

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External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Abi^Δ20

(Chen et al., 2014)

abi^Δ20

(Hamp et al., 2016, Stephan et al., 2011, Gohl et al., 2010)

abiΔ20

(Squarr et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (6)

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