UASt regulatory sequences drive expression of full-length Scr containing the mutation E19G. The protein is tagged at the N-terminal end with C-Venus, a non-fluorescent split fluorescent protein fragment. Designed to be used in a bimolecular fluorescence complementation (BiFC) assay to study protein-protein interactions in vivo.
Co-expression of ScrFL.E19G.Scer\UAS.N.T:Avic\GFP-VN and ScrFL.E19G.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4hs.PB does not result in the formation of ectopic salivary glands in the embryo.
Co-expression of ScrFL.E19G.Scer\UAS.N.T:Avic\GFP-VN and ScrFL.E19G.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4nullo.PG does not result in the transformation of mesothoracic and metathoracic segments towards prothoracic segments in the embryo.