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General Information
Symbol
Dmel\ScrSynth.DD.E19G.UAS.N.N-Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0269443
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a synthetic version of Scr consisting of the YPWM motif, the homeodomain and the small C-terminus. The sixth and seventh residues of the homeodomain have been mutated from TS to DD and the protein also contains the mutation E19G. The protein is tagged at the N-terminal end with N-Venus, a non-fluorescent split fluorescent protein fragment. Designed to be used in a bimolecular fluorescence complementation (BiFC) assay to study protein-protein interactions in vivo.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Co-expression of ScrSynth.DD.E19G.Scer\UAS.N.T:Avic\GFP-VN and ScrSynth.DD.E19G.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4hs.PB does not result in the formation of ectopic salivary glands in the embryo.

Co-expression of ScrSynth.DD.E19G.Scer\UAS.N.T:Avic\GFP-VN and ScrSynth.DD.E19G.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4nullo.PG does not result in the transformation of mesothoracic and metathoracic segments towards prothoracic segments in the embryo.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Construct for BiFC analysis (reconstitution of YFP fluorescence occurs when the T:Avic\GFPVN tag in this construct is brought together in the cell with a protein containing the T:Avic\GFPVC tag).

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ScrSynth.DD.E19G.Scer\UAS.N.T:Avic\GFP-VN
ScrSynth.DD.E19G.UAS.N.N-Venus
Name Synonyms
Secondary FlyBase IDs
    References (1)