FlyBase Allele Report: Dmel\AGO1[Q127X]

General Information

Symbol

Dmel\AGO1^Q127X

Species

D. melanogaster

Name

FlyBase ID

FBal0269731

Feature type

allele

Associated gene

Dmel\AGO1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Pressman et al., 2012)

Mutagen

ethyl methanesulfonate

(Pressman et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q127X.

(Pressman et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:13,950,735..13,950,735 [-]

Nucleotide change:

C13950735T

Reported nucleotide change:

C?T

Amino acid change:

Q127term | AGO1-PA; Q93term | AGO1-PB; Q127term | AGO1-PC; Q127term | AGO1-PD

Reported amino acid change:

Q127term

(Pressman et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | somatic clone

(Luhur et al., 2014)

lethal | embryonic stage (with AGO1^D743N)

(Pressman et al., 2012)

lethal | embryonic stage (with AGO1^E808K)

(Pressman et al., 2012)

lethal | embryonic stage (with AGO1^R937C)

(Pressman et al., 2012)

lethal | larval stage (with AGO1^D743N)

(Pressman et al., 2012)

lethal | larval stage (with AGO1^E808K)

(Pressman et al., 2012)

lethal | larval stage (with AGO1^R937C)

(Pressman et al., 2012)

lethal | pupal stage (with AGO1^D743N)

(Pressman et al., 2012)

lethal | recessive

(Pressman et al., 2012)

lethal - all die during embryonic stage

(Luhur et al., 2014)

lethal - all die during embryonic stage (with AGO1^R839X)

(Pressman et al., 2012)

some die during embryonic stage (with AGO1^R839X)

(Pressman et al., 2012)

visible | somatic clone

(Pressman et al., 2012)

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | somatic clone

(Luhur et al., 2014)

alpha/beta Kenyon cell | somatic clone

(Luhur et al., 2014)

alpha'/beta' Kenyon cell | somatic clone

(Luhur et al., 2014)

eye | somatic clone

(Pressman et al., 2012)

mushroom body | somatic clone

(Luhur et al., 2014)

Detailed Description

Statement

Reference

Homozygous AGO1^Q127X adult mushroom body clones display a reduction in cell number and absence of α'/β' and α/β neurons.

Homozygous anterodorsal projection neuron (adPN) clones show a reduction in cell number compared to controls when analysed at the adult stage. This cell number defect is not seen in homozygous adPN clones in which only early larval born adPNs are marked.

(Luhur et al., 2014)

Eyes entirely mutant for AGO1^Q127X (generated using the eyFLP method) are greatly reduced in size.

(Pressman et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

AGO1^k08121

(Pressman et al., 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

AGO1^Q127X

Ago1^Q127X

(Pressman et al., 2012)

Q127X

(Pressman et al., 2012)

ago1^Q127X

(Buddika et al., 2020, Cassidy et al., 2019, Luhur et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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