FlyBase Allele Report: Dmel\N[gt-16-20]

General Information

Symbol

Dmel\N^gt-16-20

Species

D. melanogaster

Name

FlyBase ID

FBal0269865

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{N.gt-16_20}

PBac{N.gt-16_20}

Also Known As

N^gt-16_20

Key Links

Transgenic product class

missense_variant

(Leonardi et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Leonardi et al., 2011)

Mutagen

in vitro construct

(Leonardi et al., 2011)

Progenitor genotype

N^+t40

(Leonardi et al., 2011)

Carried in construct

P{N.gt-16_20}

PBac{N.gt-16_20}

Cytology

Description

Contains serine-to-alanine mutations in the EGF repeats of region 16_20 to abolish the addition of O-glucose.

(Leonardi et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ocellar bristle | heat sensitive, with Df(1)N-54l9

(Lee et al., 2013)

postocellar bristle | heat sensitive, with Df(1)N-54l9

(Lee et al., 2013)

wing vein, with N^55e11

(Pandey et al., 2018)

wing vein L2, with Df(1)N-54l9

(Lee et al., 2013)

wing vein L4, with Df(1)N-54l9

(Lee et al., 2013)

wing vein L5, with Df(1)N-54l9

(Lee et al., 2013)

Detailed Description

Statement

Reference

N^55e11 hemizygous males bearing one copy of N^gt-16-20 show wing vein loss, as compared to controls.

(Pandey et al., 2018)

Animals lacking endogenous N (Df(1)N-54l9/Y) and rescued by one copy of N^gt-16-20 exhibit a partial loss of wing veins L2, L4 and L5 at 25[o]C, as well as head bristle defects at 30[o]C.

(Lee et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

N^gt-16-20 is a suppressor of visible | recessive phenotype of rumi^Δ26

(Leonardi et al., 2011)

N^gt-16-20 is a suppressor of lethal | heat sensitive phenotype of rumi^Δ26

(Leonardi et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

N^gt-16-20/Df(1)N-54l9 is a suppressor of wing vein phenotype of Hsap\GXYLT1^UAS.Tag:HA, Scer\GAL4^ap.PU

(Lee et al., 2013)

N^gt-16-20/Df(1)N-54l9 is a suppressor of wing margin phenotype of Hsap\XXYLT1^UAS.Tag:HA, Scer\GAL4^nub-AC-62

(Lee et al., 2013)

N^gt-16-20/Df(1)N-54l9 is a suppressor of wing vein phenotype of Hsap\XXYLT1^UAS.Tag:HA, Scer\GAL4^nub-AC-62

(Lee et al., 2013)

N^gt-16-20 is a suppressor of notal microchaeta phenotype of rumi^Δ26

(Leonardi et al., 2011)

Other

Statement

Reference

N^55e11, N^gt-16-20, Scer\GAL4^nub.PU, Xxylt^UAS.Tag:HA has wing vein phenotype

(Pandey et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

N^55e11 hemizygous males bearing one copy of N^gt-16-20 show wing vein loss, which is not suppressed by the expression of Xxylt^UAS.Tag:HA under the control of Scer\GAL4^nub.PU.

(Pandey et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

N^gt-16-20 rescues N^unspecified

(Leonardi et al., 2011)

Fails to rescue

N^gt-16-20 fails to rescue N^55e11

(Pandey et al., 2018)

Comments

N^gt-16-20 fully rescues the bristle and leg phenotypes of N^unspecified alleles at 30 [o]C.

(Leonardi et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

N^gt-16-20

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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