FlyBase Allele Report: Dmel\lqf[RNAi.UAS]

General Information

Symbol

Dmel\lqf^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0270123

Feature type

allele

Associated gene

Dmel\lqf

Associated Insertion(s)

Carried in Construct

P{UAS-lqf.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Csikós et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Csikós et al., 2009)

Mutagen

in vitro construct

(Csikós et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-lqf.RNAi}

Cytology

Description

A 605bp fragment of the fifth exon of lqf is placed in an inverted repeat downstream of UAS regulatory sequences.

(Csikós et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

lqf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | heat sensitive, with Scer\GAL4^hs.PB

(Csikós et al., 2009)

Phenotype Manifest In

nurse cell | heat sensitive, with Scer\GAL4^hs.PB

(Csikós et al., 2009)

Detailed Description

Statement

Reference

Induction of lqf^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^hs.PB) in early L2 larvae by shifting the animals from 18[o]C to 32[o]C for three days results in the absence of acridine orange-positive or LysoTracker-positive granuales in wandering animals. This treatment leads to a prolonged larval stage and results in the failure of metamorphosis. Without heat treatment, the acridine-positive and LysoTracker-positive granules appear in the trophocytes of these larvae and their development is unaffected.

(Csikós et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

lqf^RNAi.UAS

lqf^{dsRNA.Scer\UAS}

lqf^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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