Mhc promoter sequences drive expression of a fusion protein in which the FRET-based Cameleon(YC3.1) Ca2[+] sensor is localized to the postsynaptic cell membrane due to the presence of the extracellular and transmembrane domains of Hsap\CD8A at its N-terminal end and the PDZ-interaction domain of Sh at its C-terminal end.
The physiological properties of the neuromuscular junction (NMJ) are normal in larvae carrying Avic\GFPSynapcam3.1.Sh.Mhc and NMJ morphology appears normal.