UASt regulatory sequences drive expression of a 500bp inverted repeat.
Expression of CG6660NIG.6660R under the control of Scer\GAL4sal.BO results in lethality at the L2/L3 larval transition.
Larvae expressing CG6660NIG.6660R under the control of Scer\GAL4sal.BO and transferred to blue tinted food medium at the L2 or early L3 stage show blue staining of the spiracles that sometimes extends into the main tracheal trunks, indicating a water-tightness defect in the spiracles.