UASt regulatory sequences drive expression of a 500bp inverted repeat.
Scer\GAL4tub.PU-mediated expression of cv-dNIG.9622R results in almost complete loss of the posterior cross vein in adult wings: 6% have no posterior crossvein (PCV), 52% have a floating PCV, 15% have a detached PCV, 27% are normal.
Expression of cv-dNIG.9622R using Scer\GAL4tub.PU generates posterior crossvein-less adults.
Scer\GAL4hh.PU- or Scer\GAL4nub-AC-62-mediated expression of cv-dNIG.9622R does not disrupt posterior crossvein development.