UAS regulatory sequences drive expression of catalytically dead Evi5 (carries a R160A amino acid replacement), tagged at the C-terminal end with mCherry.
Expression of Evi5R160A.Scer\UAS.T:Disc\RFP-mCherry in border cells, under the control of Scer\GAL4slbo.2.6 does not affect border cell migration.
Scer\GAL4c306/Evi5R160A.UAS.mCherry fails to rescue Evi5GD6735