UASt regulatory sequences drive expression of the C-terminal domain of Cenp-C tagged at the N-terminal end with EGFP and fused to BubR1.
Embryos expressing BubR1Cenp-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 progress normally through mitosis 14.