FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\E2f1DBD.UASp.EGFP
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General Information
Symbol
Dmel\E2f1DBD.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0277720
Feature type
allele
Associated gene
Dmel\E2f1
Associated Insertion(s)
Carried in Construct
P{UASp-GFP-E2f1.DBD}
Key Links
Transgenic product class
missense_variant
(Davidson and Duronio, 2012)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Davidson and Duronio, 2012)
Mutagen
in vitro construct
(Davidson and Duronio, 2012)
Progenitor genotype
Carried in construct
P{UASp-GFP-E2f1.DBD}
Cytology
Description

UASp regulatory sequences drive expression of a mutant form of E2f1 in which four amino acids in the RRXYD motif have been replaced by alanine (R292A, R293A, Y295A, D296A). The construct is tagged with EGFP.

(Davidson and Duronio, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
E2f1
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The rate of proliferation in trypsin-dissociated posterior wing disc cells expressing E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E is similar to controls. A comparable proportion of cells have a G1 DNA content compared to controls.

      A smaller proportion of trypsin-dissociated posterior compartment cells from third instar larval wing discs expressing E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E are in S phase compared to controls.

      Expression of E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E does not induce apoptosis in the posterior compartment of intact third instar larval wing discs. The same is seen when the wing disc cells are trypsin-dissociated and quantified with flow cytometry.

      As in wild type, very few of the cells posterior to the morphogenetic furrow in eye discs expressing E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU display DNA damage and similar levels of apoptosis are seen compared with controls.

      (Davidson and Duronio, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Wing discs co-expressing E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4en-e16E appear largely normal.

      (Davidson and Duronio, 2012)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      E2f1DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP
      E2f1DBD.UASp.EGFP
      E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)