FlyBase Allele Report: Dmel\E2f1[PIP-3A-DBD.UASp.EGFP]

General Information

Symbol

Dmel\E2f1^{PIP-3A-DBD.UASp.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0277725

Feature type

allele

Associated gene

Dmel\E2f1

Associated Insertion(s)

Carried in Construct

P{UASp-GFP-E2f1.PIP-3A-DBD}

Key Links

Transgenic product class

missense_variant

(Davidson and Duronio, 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Davidson and Duronio, 2012)

Mutagen

in vitro construct

(Davidson and Duronio, 2012)

Progenitor genotype

Carried in construct

P{UASp-GFP-E2f1.PIP-3A-DBD}

Cytology

Description

UASp regulatory sequences drive expression of a mutated form of E2f1 which contains the amino acid replacements I153A, Y156A and Y157A. Four amino acids in the RRXYD motif have also been replaced by alanine (R292A, R293A, Y295A, D296A). The protein is also tagged with EGFP.

(Davidson and Duronio, 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

E2f1

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death | third instar larval stage, with Scer\GAL4^en-e16E

(Davidson and Duronio, 2012)

Phenotype Manifest In

wing disc | posterior | third instar larval stage, with Scer\GAL4^en-e16E

(Davidson and Duronio, 2012)

Detailed Description

Statement

Reference

The rate of proliferation in trypsin-dissociated posterior wing disc cells expressing E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E is similar to controls. A comparable proportion of cells have a G1 DNA content compared to controls.

Expression of E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E induces apoptosis in the posterior compartment of intact third instar larval wing discs. A similar increase in apoptosis is seen when the wing disc cells are trypsin-dissociated and quantified with flow cytometry.

The cells of the second mitotic wave in flies expressing E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU show the stereotypical pattern of S phase seen in controls. As in wild type very few cells display DNA damage, however an increase in apoptosis is seen in the cells posterior to the morphogenetic furrow.

(Davidson and Duronio, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

E2f1^{PIP-3A-DBD.UASp.EGFP}, Scer\GAL4^en-e16E has abnormal cell death | third instar larval stage phenotype, suppressible | partially by hid⁰⁵⁰¹⁴/W[+]

(Davidson and Duronio, 2012)

E2f1^{PIP-3A-DBD.UASp.EGFP}, Scer\GAL4^en-e16E has abnormal cell death | third instar larval stage phenotype, suppressible | partially by Df(3L)H99/+

(Davidson and Duronio, 2012)

Other

Statement

Reference

BacA\p35^UAS.cHa, E2f1^{PIP-3A-DBD.UASp.EGFP}, Scer\GAL4^en-e16E has viable phenotype

(Davidson and Duronio, 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

E2f1^{PIP-3A-DBD.UASp.EGFP}, Scer\GAL4^en-e16E has wing disc | posterior | third instar larval stage phenotype, suppressible | partially by hid⁰⁵⁰¹⁴/W[+]

(Davidson and Duronio, 2012)

E2f1^{PIP-3A-DBD.UASp.EGFP}, Scer\GAL4^en-e16E has wing disc | posterior | third instar larval stage phenotype, suppressible | partially by Df(3L)H99/+

(Davidson and Duronio, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of W⁰⁵⁰¹⁴ partially suppresses the apoptosis seen in the posterior compartment of third instar larval wing discs expressing E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E.

One copy of Df(3L)H99 partially suppresses the apoptosis seen in the posterior compartment of third instar larval wing discs expressing E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E.

(Davidson and Duronio, 2012)

Xenogenetic Interactions

Statement

Reference

Wing discs co-expressing E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP} and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E appear largely normal.

Wing discs co-expressing E2f^{DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP} and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E appear largely normal.

(Davidson and Duronio, 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E2f1^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}

E2f1^{PIP-3A-DBD.UASp.EGFP}

E2f^{PIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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