FlyBase Allele Report: Dmel\Fic[55]

General Information

Symbol

Dmel\Fic⁵⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0277878

Feature type

allele

Associated gene

Dmel\Fic

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Rahman et al., 2012)

Mutagen

Delta2-3 transposase

(Rahman et al., 2012)

Progenitor genotype

P{lacW}Fic^k11101

(Rahman et al., 2012)

Cytology

Description

Excision of the P{lacW} element in P{lacW}Fic^k11101 has resulted in deletion of the 5'UTR and first 260 amino acids of Fic, including the highly conserved tetratricopeptide repeat domain.

(Rahman et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:6,424,390..6,425,305 [+]

Comment:

Deletion resulting from the imprecise excision of P{lacW}Fic^k11101, which removes 5' UTR and 260 aa of Fic. Exact endpoints not specified.

(Rahman et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Rahman et al., 2012)

abnormal neurophysiology (with Df(2L)BSC296)

(Rahman et al., 2012)

abnormal visual behavior

(Rahman et al., 2012)

fertile

(Rahman et al., 2012)

viable

(Rahman et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous Fic⁵⁵ mutant flies are not attracted to the light source in phototaxis assays. This defect could not be rescued by feeding flies a diet enriched with either histamine or carcinine.

Electroretinograms show that Fic⁵⁵ mutant flies undergo normal light-induced depolarisation. However, they lack the ON transient, which reflects synaptic activation of the postsynaptic laminar neurons. Partial restoration of ON transients is achieved through feeding flies a diet enriched with either histamine or carcinine.

Electroretinograms show that Fic⁵⁵/Df(2L)BSC296 mutant flies undergo normal light-induced depolarisation. However, they lack the ON transient, which reflects synaptic activation of the postsynaptic laminar neurons.

No defects in the arrangement of photoreceptor axons or rhabdomeres are evident in Fic⁵⁵ mutants. Mitochondria, synaptic vesicles and active zone T bars also appear unchanged.

Flies containing Fic⁵⁵ mutant whole-eye clones (generated using the eyFLP method) exhibit normal ON transients as seen in controls. Response to light in phototaxis assays also appears normal.

Fic⁵⁵ mutant glial cells have a slightly reduced frequency of capitate projections compared to controls, with ~2.8 projections per synaptic cross section compared to approximately 4.3 in wild type. The shape and average diameter of the projections are similar to controls.

(Rahman et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Fic⁵⁵ is rescued by Fic^UAS.cRa/Scer\GAL4^arm.PS

(Rahman et al., 2012)

Fic⁵⁵ is rescued by Scer\GAL4^arm.PS/Fic^UAS.GFP

(Rahman et al., 2012)

Fic⁵⁵ is rescued by Fic^UAS.HRP/Scer\GAL4^arm.PS

(Rahman et al., 2012)

Fic⁵⁵ is rescued by Fic^+tRa

(Rahman et al., 2012)

Partially rescued by

Fic⁵⁵ is partially rescued by Fic^UAS.cRa/Scer\GAL4^repo

(Rahman et al., 2012)

Not rescued by

Fic⁵⁵ is not rescued by Scer\GAL4^elav-C155/Fic^UAS.cRa

(Rahman et al., 2012)

Fic⁵⁵ is not rescued by Fic^H375A.UAS/Scer\GAL4^arm.PS

(Rahman et al., 2012)

Fic⁵⁵ is not rescued by Fic^H375A.UAS/Scer\GAL4^repo

(Rahman et al., 2012)

Comments

Expression of Fic^+tRa rescues the phototaxis defects seen in homozygous Fic⁵⁵ mutant flies. The loss of the ON transients in electroretinograms is also rescued.

Expression of Fic^Scer\UAS.cRa under the control of Scer\GAL4^arm.PS rescues the loss of the ON transients in Fic⁵⁵ mutant electroretinograms.

Expression of Fic^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^arm.PS rescues the loss of the ON transients in Fic⁵⁵ mutant electroretinograms.

Expression of Fic^{Scer\UAS.T:Arus\HRP} under the control of Scer\GAL4^arm.PS rescues the loss of the ON transients in Fic⁵⁵ mutant electroretinograms.

Expression of Fic^{H375A.Scer\UAS} under the control of Scer\GAL4^arm.PS fails to rescue the loss of the ON transients in Fic⁵⁵ mutant electroretinograms.

Expression of Fic^Scer\UAS.cRa under the control of Scer\GAL4^elav-C155 fails to rescue the loss of the ON transients in Fic⁵⁵ mutant electroretinograms. The defects in phototaxis seen in Fic⁵⁵ mutants are also not rescued.

Expression of Fic^Scer\UAS.cRa under the control of Scer\GAL4^repo rescues the loss of the ON transients in Fic⁵⁵ mutant electroretinograms. The defects in phototaxis seen in Fic⁵⁵ mutants are partially rescued.

Expression of Fic^{H375A.Scer\UAS} under the control of Scer\GAL4^repo fails to rescue the loss of the ON transients in Fic⁵⁵ mutant electroretinograms. The defects in phototaxis seen in Fic⁵⁵ mutants are also not rescued.

(Rahman et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Fic⁵⁵

(Rahman et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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