UAS regulatory sequences drive expression of a mutated kirre protein that also carries a C-terminal Tag:FLAG-tag. Four putative phosphorylation sites (T637, S680, T810 and T814 ) are mutated to Alanine.
Scer\GAL4how-24B/kirreT637A.S680A.T810A.T814A.UAS.Tag:FLAG partially rescues Df(1)w67k30
Expression of kirreT637A.S680A.T810A.T814A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4how-24B only partially restores fusion competent myoblast (FCM) fusion in Df(1)w67k30 mutant embryos.